Lake Erie Center and Department of Environmental Sciences, University of Toledo, Toledo, OH 43616, USA.
Mol Phylogenet Evol. 2010 Oct;57(1):434-47. doi: 10.1016/j.ympev.2010.06.017. Epub 2010 Jul 1.
The phylogenetic systematic relationships of the enigmatic greenside darter Etheostoma blennioides complex are analysed using sequences from the mitochondrial (mt) DNA cytochrome b gene and nuclear S7 ribosomal protein intron 1 from putative members of the complex, close relatives, and outgroups (totaling 421 individuals). We compare results from Bayesian and maximum likelihood analysis approaches and a variety of rooting and taxon inclusion scenarios, and include all putative subspecies and intergrade taxa for a new comprehensive analysis. Results reveal that nuclear and mtDNA data congruently, under all scenarios and approaches tested, define a highly-supported restricted greenside darter complex comprising three putative subspecies: E. b. blennioides, E. b. pholidotum, and part of E. b. newmanii (excepting those from the Tennessee/Hiwassee River clade). Within this redefined E. blennioides, only a single putative subspecies -E. b. blennioides - is monophyletic in the mtDNA trees, and none are monophyletic in the nuclear DNA trees. Nuclear and mtDNA results support E. gutselli as a separate species and suggest that the Tennessee/Hiwassee River clade of "E. b. newmanii" also may constitute a separate species (provisionally "E. newmanii"), with neither being a part of our redefined E. blennioides complex. The nuclear DNA trees depict the two as highly-supported divergent clades, but the mtDNA results group them together as a single clade, indicating introgression. Future study with greater sample sizes in the southern watersheds, coupling morphological analyses with additional nuclear gene phylogenies, is recommended to further investigate the relationships within the greenside darter complex.
神秘的绿鳃太阳鱼复合体的系统发育关系是利用来自线粒体 (mt) DNA 细胞色素 b 基因和核 S7 核糖体蛋白内含子 1 的序列进行分析的，这些序列来自复合体的假定成员、近亲以及外群（共计 421 个个体）。我们比较了贝叶斯和最大似然分析方法以及各种生根和分类群包含情景的结果，并包括了所有假定的亚种和杂交分类单元，以进行新的综合分析。结果表明，在所有测试的情景和方法下，核 DNA 和 mtDNA 数据一致地定义了一个高度支持的受限绿鳃太阳鱼复合体，由三个假定的亚种组成：E. b. blennioides、E. b. pholidotum 和 E. b. newmanii 的一部分（不包括田纳西/海华沙河分支的那些）。在这个重新定义的 E. blennioides 中，只有一个假定的亚种——E. b. blennioides——在 mtDNA 树中是单系的，而在核 DNA 树中则没有一个是单系的。核 DNA 和 mtDNA 的结果支持 E. gutselli 是一个独立的物种，并表明田纳西/海华沙河分支的“E. b. newmanii”也可能构成一个独立的物种（暂定名为“E. newmanii”），两者都不是我们重新定义的 E. blennioides 复合体的一部分。核 DNA 树描绘了这两个高度支持的分歧分支，但 mtDNA 结果将它们分组为一个单一的分支，表明存在基因渗入。建议在南部流域进行更大规模的样本研究，将形态分析与额外的核基因系统发育相结合，以进一步研究绿鳃太阳鱼复合体内部的关系。
