Molecular Plant-Microbe Interactions Group, School of Biology and Environmental Science, University College Dublin, Science West, Belfield, Dublin-4, Ireland.
Biol Trace Elem Res. 2011 Aug;142(2):232-41. doi: 10.1007/s12011-010-8754-6. Epub 2010 Jul 3.
Iron deficiency anemia is one of the serious ailments related to nutrition in the developing countries. Fruit and vegetable crops favor the bioavailability of iron. Banana is consumed as a staple food in the tropics. Iron-fortified bananas provide an effective means of controlling the iron deficiency. Embryogenic cells of banana cv. Rasthali (AAB) were transformed with soybean ferritin cDNA using two different expression cassettes pSF and pEFE-SF to express ferritin. Transgenic nature of the regenerated plants was confirmed by PCR. Transgenic plants were regenerated and analyzed through PCR and PCR-Southern analysis. The expression of ferritin was confirmed by RT-PCR. Iron and zinc levels in the transgenic and control plants were estimated by atomic absorption spectroscopy. A 6.32-fold increase in iron accumulation and a 4.58-fold increase in the zinc levels were noted in the leaves of transgenic plants. Thus, iron- and zinc-fortified bananas could be developed as a functional food to overcome the malnutrition-related iron deficiency. This is the first report on the iron and zinc fortification of banana.
缺铁性贫血是发展中国家与营养有关的严重疾病之一。水果和蔬菜作物有利于铁的生物利用度。香蕉在热带地区被作为主食食用。铁强化香蕉提供了控制缺铁的有效手段。利用两个不同的表达盒 pSF 和 pEFE-SF 将大豆铁蛋白 cDNA 转化为香蕉 cv. Rasthali（AAB）的胚胎细胞，以表达铁蛋白。通过 PCR 确认再生植物的转基因性质。通过 PCR 和 PCR- Southern 分析对转基因植物进行再生和分析。通过 RT-PCR 确认铁蛋白的表达。通过原子吸收光谱法估计转基因和对照植物中的铁和锌含量。在转基因植物的叶片中观察到铁积累增加了 6.32 倍，锌水平增加了 4.58 倍。因此，铁和锌强化香蕉可以开发为功能性食品，以克服与营养不良有关的缺铁。这是关于香蕉铁和锌强化的第一个报告。
