Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei 10617, Taiwan.
Antiviral Res. 2010 Oct;88(1):25-30. doi: 10.1016/j.antiviral.2010.06.010. Epub 2010 Aug 23.
Feline infectious peritonitis (FIP) is a fatal disease in domestic and nondomestic felids caused by feline coronavirus (FCoV). Currently, no effective vaccine is available for the prevention of this disease. In searching for agents that may prove clinically effective against FCoV infection, 16 compounds were screened for their antiviral activity against a local FCoV strain in Felis catus whole fetus-4 cells. The results showed that Galanthus nivalis agglutinin (GNA) and nelfinavir effectively inhibited FCoV replication. When the amount of virus preinoculated into the test cells was increased to mimic the high viral load present in the target cells of FIP cats, GNA and nelfinavir by themselves lost their inhibitory effect. However, when the two agents were added together to FCoV-infected cells, a synergistic antiviral effect defined by complete blockage of viral replication was observed. These results suggest that the combined use of GNA and nelfinavir has therapeutic potential in the prophylaxis and treatment of cats with early-diagnosed FIP.
猫传染性腹膜炎（FIP）是一种由猫冠状病毒（FCoV）引起的、在家猫和非家猫中致命的疾病。目前，尚无有效的疫苗可用于预防这种疾病。在寻找可能对 FCoV 感染具有临床疗效的药物时，我们筛选了 16 种化合物，以评估它们对本地猫源 FCoV 株在猫胎儿全组织-4 细胞中的抗病毒活性。结果表明，雪花莲凝集素（GNA）和奈非那韦能有效抑制 FCoV 复制。当预先接种到测试细胞中的病毒量增加到模拟 FIP 猫靶细胞中存在的高病毒载量时，GNA 和奈非那韦本身失去了抑制作用。然而，当这两种药物一起添加到感染 FCoV 的细胞中时，观察到了完全阻断病毒复制的协同抗病毒作用。这些结果表明，联合使用 GNA 和奈非那韦在预防和治疗早期诊断为 FIP 的猫中具有治疗潜力。
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