Experimental Surgical Unit, 2nd Department of Surgery, Medical School, University of Athens, Aretaieion Hospital, Athens 11528, Greece.
World J Surg Oncol. 2010 Jul 7;8:57. doi: 10.1186/1477-7819-8-57.
The liver presents a remarkable capacity for regeneration after hepatectomy but the exact mechanisms and mediators involved are not yet fully clarified. Erythropoietin (EPO) and Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) have been shown to promote liver regeneration after major hepatectomy.Aim of this experimental study is to compare the impact of exogenous administration of EPO, GM-CSF, as well as their combination on the promotion of liver regeneration after major hepatectomy.
Wistar rats were submitted to 70% major hepatectomy. The animals were assigned to 4 experimental groups: a control group (n = 21) that received normal saline, an EPO group (n = 21), that received EPO 500 IU/kg, a GM-CSF group (n = 21) that received 20 mcg/kg of GM-CSF and a EPO+GMCSF group (n = 21) which received a combination of the above. Seven animals of each group were killed on the 1st, 3rd and 7th postoperative day and their remnant liver was removed to evaluate liver regeneration by immunochemistry for PCNA and Ki 67.
Our data suggest that EPO and GM-CSF increases liver regeneration following major hepatectomy when administered perioperatively. EPO has a more significant effect than GM-CSF (p < 0.01). When administering both, the effect of EPO seems to fade as EPO and GM-CSF treated rats have decreased regeneration compared to EPO administration alone (p < 0.01).
EPO, GM-CSF and their combination enhance liver regeneration after hepatectomy in rats when administered perioperatively. However their combination has a weaker effect on liver regeneration compared to EPO alone. Further investigation is needed to assess the exact mechanisms that mediate this finding.
肝脏在肝切除后具有显著的再生能力，但涉及的具体机制和介质尚未完全阐明。促红细胞生成素（EPO）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）已被证明可促进大肝切除后的肝再生。本实验研究的目的是比较外源性给予 EPO、GM-CSF 及其组合对大肝切除后促进肝再生的影响。
Wistar 大鼠接受 70%的大肝切除术。将动物分为 4 个实验组：对照组（n = 21）接受生理盐水，EPO 组（n = 21）接受 500 IU/kg 的 EPO，GM-CSF 组（n = 21）接受 20 mcg/kg 的 GM-CSF，EPO+GMCSF 组（n = 21）接受上述组合。每组 7 只动物分别于术后第 1、3 和 7 天处死，取出残余肝脏，通过 PCNA 和 Ki67 的免疫组化评估肝再生情况。
我们的数据表明，EPO 和 GM-CSF 在围手术期给药时可增加大肝切除术后的肝再生。EPO 的作用比 GM-CSF 更显著（p < 0.01）。同时给予两者时，EPO 和 GM-CSF 治疗的大鼠与单独给予 EPO 相比，再生能力下降，EPO 的作用似乎减弱（p < 0.01）。
EPO、GM-CSF 及其组合在围手术期给药时可增强大鼠肝切除后的肝再生。然而，与单独给予 EPO 相比，它们的组合对肝再生的作用较弱。需要进一步研究以评估介导这一发现的确切机制。
