Di Renzo L, Yefenof E, Klein E
Department of Tumor Biology, Karolinska Institutet, Stockholm, Sweden.
Eur J Immunol. 1991 Jul;21(7):1755-8. doi: 10.1002/eji.1830210726.
Cells responsible for the natural killer (NK) effect in the human blood can be collected in the low-density lymphocyte subset and the majority of them express CR3. In addition to the iC3b binding site the CR3 molecules possess an epitope which binds beta-glucan. Ligands of this site can deliver activation signals to CR3-carrying monocytes and neutrophils. We found that the function of NK cells was also potentiated by preincubation with beta-glucan. The treatment increased the proportion of target-binding lymphocytes and of the damaged target cells in the conjugates. The monoclonal antibody OKM-1, directed to the beta-glucan-binding site of CR3, abrogated this effect. Another CR3-reactive monoclonal antibody, M522, known to activate monocytes and neutrophils, enhanced the NK function.
负责人类血液中自然杀伤（NK）效应的细胞可在低密度淋巴细胞亚群中收集，其中大多数表达CR3。除了iC3b结合位点外，CR3分子还拥有一个与β-葡聚糖结合的表位。该位点的配体可向携带CR3的单核细胞和中性粒细胞传递激活信号。我们发现，用β-葡聚糖预孵育也可增强NK细胞的功能。这种处理增加了结合靶细胞的淋巴细胞比例以及结合物中受损靶细胞的比例。针对CR3的β-葡聚糖结合位点的单克隆抗体OKM-1消除了这种效应。另一种已知可激活单核细胞和中性粒细胞的CR3反应性单克隆抗体M522增强了NK功能。
