棕榈酰肉碱对分离的豚鼠心室肌细胞被动电特性的影响。

Effect of palmitoylcarnitine on the passive electrical properties of isolated guinea pig ventricular myocytes.

作者信息

Mèszàros J

机构信息

Department of Pharmacology, University of Connecticut Health Center, Farmington 06032.

出版信息

Eur J Pharmacol. 1991 Feb 26;194(1):107-10. doi: 10.1016/0014-2999(91)90130-i.

DOI:10.1016/0014-2999(91)90130-i

PMID:2060586

Abstract

The effects of palmitoylcarnitine (PC) on the passive electrical properties of isolated guinea pig ventricular myocytes were studied using conventional microelectrode techniques. The amphiphile depolarized the cell membrane, and at low concentration (10(-6) M), increased the input resistance (Rin) from 12.4 to 17.9 M omega and the time constant (tau m) from 1.38 to 2.20 ms. A higher concentration of PC (10(-5) M) decreased Rin to 8.5 M omega and tau m to 1.14 ms. No significant change was observed in the membrane capacitance and in the capacitative membrane area. The results suggest that PC incorporates into the cell membrane and in low concentration increases the membrane resistance, while its high concentration increases the membrane permeability probably by causing serious damages in the membrane structure.

摘要

采用传统微电极技术研究了棕榈酰肉碱（PC）对分离的豚鼠心室肌细胞被动电特性的影响。这种两亲性物质使细胞膜去极化，在低浓度（10^(-6) M）时，输入电阻（Rin）从12.4 MΩ增加到17.9 MΩ，时间常数（τm）从1.38 ms增加到2.20 ms。较高浓度的PC（10^(-5) M）使Rin降至8.5 MΩ，τm降至1.14 ms。膜电容和电容性膜面积未观察到显著变化。结果表明，PC掺入细胞膜，低浓度时增加膜电阻，而高浓度时可能通过对膜结构造成严重损伤增加膜通透性。