Department of Physiology, Faculty of Medicine and Dentistry, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain.
Histol Histopathol. 2010 Sep;25(9):1133-8. doi: 10.14670/HH-25.1133.
The presence of CB1 and CB2 cannabinoid receptors and their physiological role in the kidney has been described in animal models but not in humans. Our aim in this study was to evaluate the presence of these receptors in human kidney, adult and fetal. For this purpose, RT-PCR, western-blot and immunohisto-chemical assays were performed. RT-PCR confirmed the presence of CB1 receptor mRNA receptor and the absence of the CB2 receptor mRNA in adult and fetal kidney. Western-blot and immunohistochemical assays revealed the presence of the CB1 cannabinoid receptor protein, which displayed a similar distribution in fetal and adult kidneys. Proximal and distal convoluted tubule cells and intercalated cells in the collecting ducts showed marked positivity. Conversely, the CB2 cannabinoid receptor protein was consistently negative in all cases. Our data suggest a possible implication of the endocannabinoid system in the physiology and development of the human kidney.
在动物模型中已经描述了 CB1 和 CB2 大麻素受体及其在肾脏中的生理作用，但在人类中尚未描述。我们在这项研究中的目的是评估这些受体在人类肾脏、成人和胎儿中的存在。为此，进行了 RT-PCR、western-blot 和免疫组织化学检测。RT-PCR 证实了成人和胎儿肾脏中 CB1 受体 mRNA 受体的存在和 CB2 受体 mRNA 的缺失。Western-blot 和免疫组织化学检测显示存在 CB1 大麻素受体蛋白，其在胎儿和成人肾脏中的分布相似。近端和远端曲管细胞以及集合管中的闰细胞显示出明显的阳性。相反，在所有情况下 CB2 大麻素受体蛋白均呈阴性。我们的数据表明内源性大麻素系统可能参与了人类肾脏的生理和发育。
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