First Propaedeutic Surgical Department, Hippocration University Hospital, University of Athens, Athens, Greece.
J Surg Oncol. 2010 Nov 1;102(6):604-7. doi: 10.1002/jso.21648.
E-selectin, an intercellular adhesion molecule that plays important roles in metastasis of tumor cells, has a polymorphism in exon 4 that results in the substitution of a serine by an arginine within the extracellular domain of the receptor, which increases its affinity for ligands. No evidence exist on the role of E-selectin polymorphism in pancreatic cancer.
Eighty pancreatic cancer patients and 160 cases of normal healthy control subjects were investigated for genotype and allelic frequencies of S128R polymorphism of E-selectin gene by PCR-RFLPs.
The frequencies for "AA," "CA," and "CC" genotypes were 46.25%, 50%, and 3.75% in patients, and 63.75%, 26.9%, and 9.4% in healthy subjects, respectively. The "C" carriers group of patients ("CA + CC" genotype) and the "C" allele were over-represented among the pancreatic cancer cases (P = 0.012 and 0.096, respectively). Advanced T stage, the presence of lymph node and other adverse pathologic characteristics were not significantly correlated with either the "CA + CC" genotype group of patients or the presence of "C" allele.
E-selectin S128R "C" allele may confer an increased susceptibility to pancreatic cancer development, while its carriage status does not appear to be related to the aggressive features of this malignancy.
E-选择素是一种细胞间黏附分子，在肿瘤细胞转移中发挥重要作用，其外显子 4 中的一个多态性导致受体细胞外结构域中丝氨酸被精氨酸取代，从而增加了其与配体的亲和力。目前尚无关于 E-选择素多态性在胰腺癌中作用的证据。
通过 PCR-RFLPs 方法，对 80 例胰腺癌患者和 160 例正常健康对照者的 E-选择素基因 S128R 多态性的基因型和等位基因频率进行了研究。
患者中“AA”、“CA”和“CC”基因型的频率分别为 46.25%、50%和 3.75%，健康对照组分别为 63.75%、26.9%和 9.4%。患者中“C”携带者组（“CA+CC”基因型）和“C”等位基因的频率均高于胰腺癌病例（P=0.012 和 0.096）。晚期 T 分期、淋巴结转移和其他不良病理特征与患者的“CA+CC”基因型组或“C”等位基因均无显著相关性。
E-选择素 S128R“C”等位基因可能增加了胰腺癌发生的易感性，但其携带状态似乎与这种恶性肿瘤的侵袭性特征无关。
