St Clair D K, Oberley L W
Department of Radiology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27103.
Free Radic Res Commun. 1991;12-13 Pt 2:771-8. doi: 10.3109/10715769109145858.
The level of manganese superoxide dismutase (MnSOD) as determined by an immunoreactive assay was reduced in human cancer cells. The reduced amount of immunoreactive MnSOD in these cells was observed regardless of the growth state of the cells. The decrease in the enzyme protein was associated with a decrease in the mature form of MnSOD transcript as determined by Northern Analysis. The decreased amount of the mature form of MnSOD transcript was accompanied by an increase in the amount of the partially processed form of MnSOD transcript. These results suggest that RNA processing or translation of MnSOD mRNA may be responsible for the decreased amount of MnSOD activity in human tumor cells.
通过免疫反应性测定法测定，人类癌细胞中锰超氧化物歧化酶（MnSOD）的水平降低。无论细胞的生长状态如何，这些细胞中免疫反应性MnSOD的量均减少。通过Northern分析确定，酶蛋白的减少与MnSOD转录本成熟形式的减少有关。MnSOD转录本成熟形式的量减少伴随着MnSOD转录本部分加工形式的量增加。这些结果表明，MnSOD mRNA的RNA加工或翻译可能是人类肿瘤细胞中MnSOD活性降低的原因。
