采用异黄酮评价葛根药材质量。

Evaluation of quality of Radix Puerariae herbal medicine by isoflavonoids.

机构信息

Department of Food Science, National Chia-Yi University, Chia-Yi.

出版信息

J Pharm Pharmacol. 2010 May;62(5):644-50. doi: 10.1211/jpp.62.05.0013.

DOI:10.1211/jpp.62.05.0013

PMID:20609068

Abstract

OBJECTIVES

A high-performance liquid chromatography (HPLC) method was developed to examine five isoflavonoids present in Chinese herbal medicinal products containing Radix Puerariae.

METHODS

Five isoflavonids, puerarin, daidzin, genistin, daidzein and genistein, were measured by HPLC. The HPLC system was equipped with an ODS-AM-303 column (250 mm x 4.6 mm i.d., 5 microm) and established gradient system comprising glacial acetic acid/water and glacial acetic acid/acetonitrile.

KEY FINDINGS

The developed HPLC system yielded good separation of the five isoflavonoids. Relative coefficients of intraday and interday analysis of variation were less than 5%. The isoflavonoid recovery from Radix Puerariae was 90-113%. Most of the Radix Puerariae products studied contained five isoflavonoids in their HPLC fingerprint. The major component was purarine, then daidzin and daidzein; genistin and genistein were the least abundant. Five Radix Puerariae herbal medicines contained various concentrations of isoflavonoids. Of the 11 scientific extracted formulas of Radix Puerariae tested, ST brands had a greater isoflavonoid content than KA and SC brands.

CONCLUSIONS

Separation and quantification of the five isoflavonoids by this HPLC method was suitable to assess the quality of Radix Puerariae herbal medicine products.

摘要

目的

建立了一种高效液相色谱（HPLC）法，用于检测葛根药材中 5 种异黄酮的含量。

方法

采用 HPLC 法测定 5 种异黄酮，葛根素、大豆苷、大豆苷元、染料木素和染料木苷。HPLC 系统配备 ODS-AM-303 柱（250mm×4.6mm i.d.，5μm），并建立了包含冰醋酸/水和冰醋酸/乙腈的梯度系统。

关键发现

所建立的 HPLC 系统可实现 5 种异黄酮的良好分离。日内和日间变异的相对系数均小于 5%。葛根素的异黄酮回收率为 90-113%。研究的大部分葛根产品在其 HPLC 指纹图谱中均含有 5 种异黄酮。主要成分为葛根素，其次是大豆苷元和染料木苷；大豆苷和染料木苷的含量最少。5 种葛根草药含有不同浓度的异黄酮。在所测试的 11 种葛根科学提取配方中，ST 品牌的异黄酮含量高于 KA 和 SC 品牌。