Hubrecht Institute-KNAW and University Medical Centre, Utrecht, the Netherlands.
Development. 2010 Aug;137(16):2653-7. doi: 10.1242/dev.048207. Epub 2010 Jul 7.
The endothelial cells of the vertebrate lymphatic system assemble into complex networks, but local cues that guide the migration of this distinct set of cells are currently unknown. As a model for lymphatic patterning, we have studied the simple vascular network of the zebrafish trunk consisting of three types of lymphatic vessels that develop in close connection with the blood vasculature. We have generated transgenic lines that allow us to distinguish between arterial, venous and lymphatic endothelial cells (LECs) within a single zebrafish embryo. We found that LECs migrate exclusively along arteries in a manner that suggests that arterial endothelial cells serve as the LEC migratory substrate. In the absence of intersegmental arteries, LEC migration in the trunk is blocked. Our data therefore demonstrate a crucial role for arteries in LEC guidance.
脊椎动物淋巴系统的内皮细胞会组装成复杂的网络，但目前还不清楚是什么局部信号引导这一独特细胞群的迁移。作为淋巴系统模式形成的一个模型，我们研究了斑马鱼躯干中简单的血管网络，其中包含三种类型的淋巴管，它们与血管系统紧密相连。我们已经生成了转基因系，使我们能够在单个斑马鱼胚胎中区分动脉、静脉和淋巴管内皮细胞（LEC）。我们发现 LEC 仅沿着动脉迁移，这表明动脉内皮细胞是 LEC 迁移的基质。在没有节间动脉的情况下，躯干中的 LEC 迁移被阻断。因此，我们的数据表明动脉在 LEC 引导中起着至关重要的作用。
