Caplan R H, Koob L, Abellera R M, Pagliara A S, Kovacs K, Randall R V
Am J Med. 1978 May;64(5):874-82. doi: 10.1016/0002-9343(78)90531-4.
We studied a 30 year old woman in whom acromegaly was cured by operative removal of a large cystic beta cell adenoma of the pancreas. We detected substantial amounts of immunoreactive human growth hormone (hGH)-like activity in a tumor tissue extract. Extracts of the tumor and a normal human pituitary gland eluted from a Sephadex G-75 column in two identical peaks. Serial dilutions of the tumor extract displaced radioactive 125I hGH parallel to a standard curve. Surprisingly, an extract of a normal human pancreas contained large amounts of hGH-like activity and gave results similar to those of the tumor extract on gel chromatography and on serial dilution displacement in the growth hormone immunoassay. Paper electrophoretic studies of 125I hGH after incubation with normal pancreatic and tumor extracts with and without enzyme inhibitors suggested that pancreatic proteolytic enzymes damaged the 125I hGH used in growth hormone radioimmunoassay and produced a false detection of hGH.
我们研究了一名30岁的女性，其肢端肥大症通过手术切除胰腺的一个大型囊性β细胞腺瘤得以治愈。我们在肿瘤组织提取物中检测到大量免疫反应性人类生长激素（hGH）样活性。肿瘤提取物和正常人垂体提取物从Sephadex G - 75柱上洗脱时呈现出两个相同的峰。肿瘤提取物的系列稀释液与放射性125I hGH的结合平行于标准曲线。令人惊讶的是，正常人胰腺提取物含有大量hGH样活性，并且在凝胶色谱和生长激素免疫测定中的系列稀释置换实验中给出了与肿瘤提取物相似的结果。在用正常胰腺和肿瘤提取物孵育125I hGH后进行的纸电泳研究，无论有无酶抑制剂，结果表明胰腺蛋白水解酶会破坏生长激素放射免疫测定中使用的125I hGH，并导致hGH的假阳性检测。
