Brusentsov I I, Katokhin A V, Sakharovskaia Z V, Sazonov A E, Ogorodova L M, Fedorova O S, Kolchanov N A, Mordvinov V A
Med Parazitol (Mosk). 2010 Apr-Jun(2):10-3.
Opisthorchiasis is one of the significant naturofocal diseases in Russia. The diagnosis of opisthorchiasis may mask some diseases, such as opisthorchiasis, metorchiasis, and clonorchiasis - biohelminthoses that are induced by various representatives of the family Opisthorchiidae-Opisthorchis felineus/O.viverrini, Metorchis bilis, and Clonorchis sinensis, respectively. Coproovoscopy and serologic methods fail to accurately define the species-specific affiliation; in this connection the identification of opisthorchids, by using DNA diagnostic techniques, becomes urgent. The present paper gives the results of development of DNA diagnosticum, which differentiates parasitic diseases induced by O. felineus and M. bills. The ribosomal RNA gene cluster fragment incorporating internal transcribed spacer 2 (ITS2) was used as a diagnostic marker. A system for diagnosing opisthorchiasis was developed as a multiplex polymerase chain analysis and tested on 37 patients infected with various species of opisthorchids.
后睾吸虫病是俄罗斯重要的自然疫源性疾病之一。后睾吸虫病的诊断可能会掩盖一些疾病，如后睾吸虫病、间睾吸虫病和华支睾吸虫病——分别由后睾科的不同代表物种引起的生物源性蠕虫病，即猫后睾吸虫/麝猫后睾吸虫、胆间睾吸虫和中华支睾吸虫。粪便显微镜检查和血清学方法无法准确确定物种特异性归属；因此，利用DNA诊断技术鉴定后睾吸虫变得十分迫切。本文给出了DNA诊断试剂的研发结果，该试剂可区分由猫后睾吸虫和胆间睾吸虫引起的寄生虫病。包含内部转录间隔区2（ITS2）的核糖体RNA基因簇片段被用作诊断标记。开发了一种用于诊断后睾吸虫病的系统，作为多重聚合酶链分析，并在37例感染不同种类后睾吸虫的患者身上进行了测试。
