Department of Molecular Pathology, Faculty of Medicine, Oita University, Yufu City, Japan.
Pathobiology. 2010;77(4):191-9. doi: 10.1159/000292652. Epub 2010 Jul 7.
The aim of the present study was to clarify the expression of uridine diphospho-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE) protein and mRNA in damaged or regenerating myofibers.
We investigated the muscle expression pattern of GNE protein by immunohistochemistry using a murine model involving intramuscular injection of cardiotoxin (CTX), and the expression level of GNE mRNA by quantitative real-time polymerase chain reaction analysis of damaged or regenerating myofibers that had been collected directly from tissue sections using laser-capture microdissection.
The expression of GNE protein was increased in severely damaged myofibers as well as in regenerating myofibers with central nuclei, both of which also showed an increase in the expression of GNE mRNA. In regenerating myofibers, immunoreactivity for GNE protein in nuclei relative to that in the cytoplasm was higher at 7 days than at 4 days after CTX injection.
Our findings suggest that GNE expression is induced when myofibers are damaged or regenerating, and that GNE plays a role in muscle regeneration.
本研究旨在阐明尿苷二磷酸-N-乙酰葡糖胺 2-差向异构酶/N-乙酰甘露糖胺激酶（GNE）蛋白和 mRNA 在受损或再生肌纤维中的表达。
我们通过使用心肌毒素（CTX）肌内注射的小鼠模型，用免疫组织化学方法研究 GNE 蛋白的肌肉表达模式，并使用激光捕获显微切割直接从组织切片中收集受损或再生肌纤维，通过定量实时聚合酶链反应分析来检测 GNE mRNA 的表达水平。
在严重受损的肌纤维以及具有中央核的再生肌纤维中，GNE 蛋白的表达增加，GNE mRNA 的表达也增加。在再生肌纤维中，CTX 注射后 7 天，GNE 蛋白在细胞核中的免疫反应性相对于细胞质中的免疫反应性更高。
我们的研究结果表明，当肌纤维受损或再生时，GNE 的表达被诱导，并且 GNE 在肌肉再生中发挥作用。
