Kiseleva E M, Mirgorodskaia O A, Momot N N, Avizhenis V Iu, Moskvichev B V
Prikl Biokhim Mikrobiol. 1977 Jul-Aug;13(4):577-80.
By fractionation of purine compounds (sorbtion on the cation exchange resin KU-2 (H+), extraction, precipitation of purine compounds as Ag-complexes) a "purine fraction" of the culture liquid epiphytic bacteria No. 703 isolated from barley overground organs was obtained. The presence of isopentenyl cytokinins was demonstrated by quality reactions of the purine fraction with aromatic amines and phenols as well as by the values of Rf and UV spectrum of individual compounds examined by paper chromatography of the purine fraction.
通过对嘌呤化合物进行分级分离(吸附于阳离子交换树脂KU-2(H+)上、萃取、将嘌呤化合物沉淀为银络合物),从大麦地上器官分离得到的703号附生细菌培养液的“嘌呤组分”。通过嘌呤组分与芳香胺和酚类的定性反应以及嘌呤组分纸层析所检测的各个化合物的比移值和紫外光谱,证实了异戊烯基细胞分裂素的存在。