肥大细胞脱颗粒增强外源性树突状细胞归巢至引流淋巴结

[Mast cell degranulation enhances exogenous dendritic cells homing to draining lymph nodes].

作者信息

Ren Shu-rong, Xu Li-bin, Wu Zhi-yuan, Qu Chun-feng, Gao Mei-hua, Du Jun

机构信息

Department of Immunology, Qingdao University Medical College, Qingdao 266071, China.

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2010 Jul;26(7):638-41.

PMID:20619086

Abstract

AIM

To establish a method of enhancing exogenous bone marrow derived dendritic cells (BM-DC) homing to draining lymph nodes by induction of local mast cell degranulation.

METHODS

Compound 48/80 (c48/80) was injected into C57BL/6 scapular skin to induce local mast cell degranulation. BM-DC generated from bone marrow of syngenic mice were labeled with YG-Microspheres and injected into c48/80 or normal saline treated scapular skin. Cells derived from draining lymph nodes (DLN) were pooled together 48 h after BM-DC injection and stained with conjugated anti-CD11c. The efficiency of BM-DC homing to lymph nodes was analyzed by FCM.

RESULTS

Mast cell degranulation was locally boosted by c48/80 injection and caused enhancement of the homing of exogenous BM-DC to DLN, with an increase of 67%+/-43%. The total cells in lymph nodes also increased significantly. The fold of increase was 55%+/-43%.

CONCLUSION

Exogenous BM-DC homing to DLN can be boosted by inducing local skin mast cell degranulation.

摘要

目的

建立一种通过诱导局部肥大细胞脱颗粒来增强外源性骨髓来源树突状细胞（BM-DC）归巢至引流淋巴结的方法。

方法

将化合物48/80（c48/80）注射到C57BL/6小鼠肩胛部皮肤以诱导局部肥大细胞脱颗粒。用YG微球标记同基因小鼠骨髓产生的BM-DC，并将其注射到经c48/80或生理盐水处理的肩胛部皮肤中。BM-DC注射48小时后，收集来自引流淋巴结（DLN）的细胞，并用偶联抗CD11c进行染色。通过流式细胞术分析BM-DC归巢至淋巴结的效率。