Department of Biochemistry 280, NCMLS, Radboud University Nijmegen Medical Centre, Nijmegen 6500 HB, The Netherlands.
Acta Biomater. 2010 Dec;6(12):4666-74. doi: 10.1016/j.actbio.2010.06.038. Epub 2010 Jul 7.
There is a consistent need for a suitable natural biomaterial to function as an arterial prosthesis in achieving arterial regeneration. Natural grafts are generally obtained by decellularization of native blood vessels, but batch to batch variations may occur and the nature/content of remaining contaminants is generally unknown. In this study we fabricated a molecularly defined natural arterial graft from scratch resembling the native three layered architecture from the fibrillar extracellular matrix components collagen and elastin. Using casting, moulding, freezing and lyophilization techniques, a triple layered construct was prepared consisting of an inner layer of elastin fibres, a middle (porous) film layer of collagen fibrils and an outer scaffold layer of collagen fibrils. The construct was carbodiimide cross-linked and heparinized. Characterization included biochemical/biophysical analyses, scanning electron microscopy, micro-computed tomography, (immuno)histology and haemocompatibility. Burst pressures were up to 400mm Hg and largely conferred by the intermediate porous collagen film layer. The highly purified type I collagen fibrils and elastin fibres used did not evoke platelet aggregation in vitro. Suturability of the graft in end to side anastomosis was successful and considered adequate for in vivo application.
在实现动脉再生的过程中，始终需要一种合适的天然生物材料作为动脉假体。天然移植物通常通过对天然血管进行脱细胞化获得，但批次间可能会存在差异，且剩余污染物的性质/含量通常是未知的。在这项研究中，我们从头开始制造了一种分子定义的天然动脉移植物，其类似于源自纤维细胞外基质成分胶原蛋白和弹性蛋白的天然三层结构。使用浇铸、成型、冷冻和冻干技术，制备了由弹性纤维内层、胶原蛋白原纤维中间（多孔）膜层和胶原蛋白原纤维外层支架层组成的三层结构。该结构经过碳化二亚胺交联和肝素化处理。表征包括生化/物理分析、扫描电子显微镜、微计算机断层扫描、（免疫）组织学和血液相容性。爆破压力高达 400mmHg，主要由中间多孔胶原蛋白膜层提供。使用的高度纯化的 I 型胶原蛋白原纤维和弹性纤维在体外不会引起血小板聚集。移植物的端侧吻合缝合的可缝合性成功，被认为足以满足体内应用的需求。
