Analytical and Electrochemistry Research Unit, Chemistry Department, Faculty of Science, Tanta University, 31527-Tanta, Egypt.
Bioelectrochemistry. 2010 Oct;79(2):241-7. doi: 10.1016/j.bioelechem.2010.06.005. Epub 2010 Jun 18.
Utilizing the fascinating strong adsorptive ability, high chemical and mechanical stability properties of montmorillonite-calcium (MMT-Ca) clay, a MMT-Ca modified carbon paste electrode was developed for the sensitive determination of methocarbamol. Methocarbamol has been oxidized in buffered solutions at the developed MMT-Ca-modified CPE in a single 2-electron irreversible anodic peak. A simple and sensitive square-wave adsorptive anodic stripping voltammetric method was optimized for determination of methocarbamol in bulk form, pharmaceutical formulation and in spiked human serum using the developed modified CPE. This was carried out without the necessity for samples pretreatment and/or time-consuming liquid-liquid or solid-phase extraction steps prior to the analysis. The developed electrode exhibited an excellent sensitivity and selectivity towards methocarbamol even in the presence of 10(2)-10(4)-fold of its active ingredient "ibuprofen," common excipients, common metal ions or co-administrated drugs. Limits of detection of 3 x 10 (-9) and 1.2 x 10(-8) M and limits of quantitation of 1 x 10(-8), and 4 x 10(-8) M methocarbamol were achieved in the bulk form and in spiked human serum, respectively. Moreover, the developed method was successfully applied for determination of methocarbamol in human plasma of subjects following administration of an oral dose of Ibuflex tablets.
利用蒙脱土-钙（MMT-Ca）粘土令人着迷的强吸附能力和高化学及机械稳定性，开发了一种 MMT-Ca 修饰碳糊电极，用于灵敏测定美索巴莫。在开发的 MMT-Ca 修饰 CPE 中，美索巴莫在缓冲溶液中氧化，产生单一的 2 电子不可逆阳极峰。采用优化后的简单灵敏的方波吸附阳极溶出伏安法，在不进行样品预处理和/或耗时的液-液或固-液萃取步骤的情况下，用于测定块状、药物制剂和加标人血清中美索巴莫。在存在 10(2)-10(4)倍其活性成分“布洛芬”、常见赋形剂、常见金属离子或共同给药药物的情况下，该电极对美索巴莫具有出色的灵敏度和选择性。在块状和加标人血清中，美索巴莫的检测限分别为 3 x 10(-9)和 1.2 x 10(-8) M，定量限分别为 1 x 10(-8)和 4 x 10(-8) M。此外，该方法成功应用于测定口服 Ibuflex 片后受试者人血浆中的美索巴莫。
