Yue L, Xia Q, Luo G H, Lu Y P
Transplantation Institution, West China Hospital, Cheng Du, Si Chuan, China.
Transplant Proc. 2010 Jun;42(5):1875-80. doi: 10.1016/j.transproceed.2009.11.041.
This study sought to determine whether urinary connective tissue growth factor (CTGF) was a molecular marker for chronic allograft nephropathy (CAN).
F344 rat renal grafts orthotopically transplanted into Lewis rats following the procedure of Kamada were harvested at 4,8,12, or 16 weeks. Morphological changes were studied using hematoxylin eosin (HE) and Masson trichrome stains. Serum creatinine (SCr) was measured. CAN grades were evaluated according to the Banff97 schema. Expressions of CTGF in the kidney and urine were determined using real-time polymerase chain reaction (PCR) Western blots, and competitive indirect enzyme-linked immunosorbent assay (ELISA). Spearman correlation analysis was used to compare urinary CTGF expression and CAN development.
SCr levels and Banff scores increased in a time-dependent manner. The expression of CTGF in the graft was markedly elevated compared with the control group. Urine CTGF increased by week 4, and maintained high levels up to week 16. The urinary levels correlated positively with the histological presence of CAN. Thus, urine CTGF concentrations reflected the course of CAN, especially at an early stage.
CTGF plays a significant role in the pathological changes of CAN after kidney transplantation. Urinary CTGF has the potential to be a biomarker for CAN.
本研究旨在确定尿结缔组织生长因子（CTGF）是否为慢性移植肾肾病（CAN）的分子标志物。
按照Kamada的方法将F344大鼠肾移植到Lewis大鼠体内，分别于4、8、12或16周时取移植肾。采用苏木精-伊红（HE）染色和Masson三色染色研究形态学变化。测定血清肌酐（SCr）。根据Banff97标准评估CAN分级。采用实时聚合酶链反应（PCR）、蛋白质免疫印迹法和竞争性间接酶联免疫吸附测定（ELISA）法检测肾组织和尿液中CTGF的表达。采用Spearman相关分析比较尿CTGF表达与CAN进展情况。
SCr水平和Banff评分呈时间依赖性升高。与对照组相比，移植肾中CTGF的表达明显升高。尿CTGF在第4周时升高，并在第16周前维持在较高水平。尿CTGF水平与CAN的组织学表现呈正相关。因此，尿CTGF浓度反映了CAN的病程，尤其是在早期。
CTGF在肾移植后CAN的病理变化中起重要作用。尿CTGF有可能成为CAN的生物标志物。
