植物 RING 指蛋白对烟草花叶病毒感染的抑制作用。
Inhibitory effect on the tobacco mosaic virus infection by a plant RING finger protein.
机构信息
Laboratory of Plant Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
出版信息
Virus Res. 2010 Oct;153(1):50-7. doi: 10.1016/j.virusres.2010.07.005. Epub 2010 Jul 17.
In the yeast two-hybrid screening of plant factors interacting with tobacco mosaic virus (TMV) RNA-dependent RNA polymerase (RdRp), we found a protein containing a RING finger motif in tobacco (Nicotiana tabacum) and designated it as TARF (TMV-associated RING finger protein). TARF is a homologue of a Lotus japonicus RING finger protein (LjnsRING) involved in the symbiotic interaction between L. japonicus and Mesorhizobium loti. When TARF was silenced by virus-induced gene silencing (VIGS) method, TMV RNA accumulation as well as the number of foci formed by GFP-tagged TMV increased drastically. Transient overexpression of TARF reduced the accumulation of TMV. Moreover, TARF transcription was rapidly upregulated by the inoculation of TMV in tobacco plants. These results indicated that TARF is a RING finger protein that inhibits the accumulation of TMV via the interaction of TMV RdRp.
在利用酵母双杂交筛选与烟草花叶病毒（TMV）RNA 依赖性 RNA 聚合酶（RdRp）互作的植物因子的过程中，我们在烟草（Nicotiana tabacum）中发现了一个含有 RING 指结构域的蛋白，并将其命名为 TARF（TMV 相关的 RING 指蛋白）。TARF 是参与大豆（Lotus japonicus）与根瘤菌（Mesorhizobium loti）共生互作的大豆 RING 指蛋白（LjnsRING）的同源物。当利用病毒诱导的基因沉默（VIGS）方法沉默 TARF 时，TMV RNA 的积累以及 GFP 标记的 TMV 形成焦点的数量急剧增加。TARF 的瞬时过表达则降低了 TMV 的积累。此外，在烟草植物中接种 TMV 可快速上调 TARF 的转录。这些结果表明，TARF 是一种 RING 指蛋白，它通过与 TMV RdRp 的相互作用抑制 TMV 的积累。
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