Division of Biosystems Research, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea.
J Microbiol Biotechnol. 2010 Jun;20(6):1027-31. doi: 10.4014/jmb.1002.02028.
To examine the possibility of horizontal gene transfer between transgenic potatoes and microorganisms in potato fields, the gene flow from transgenic potatoes containing nucleoside diphosphate kinase 2 (NDPK2) gene to microorganisms in soils was investigated. The soil samples collected from the potato fields from March to October in 2007 were examined by PCR, Southern hybridization, and AFLP fingerprinting. The NDPK2 gene from soil genomic DNAs was not detected by both PCR and Southern hybridization, indicating that gene-transfer did not occur in the potato fields. In addition, no discrepancy was found in pathogenicity and noticeable changes for the appearance of variants of Phytophthora infestans in each generation when serial inoculations and the analysis of genomic DNAs by AFLP was conducted. Thus, these data suggest that transgenic potatoes do not give significant impacts on the communities of soil microorganisms and the emergence of variants although continued research efforts may be necessary to make a decisive conclusion.
为了研究转基因马铃薯与田间土壤微生物之间是否存在水平基因转移的可能性，本研究调查了含核苷二磷酸激酶 2（NDPK2）基因的转基因马铃薯向土壤微生物的基因流。从 2007 年 3 月至 10 月采集的田间土壤样品，经 PCR、Southern 杂交和 AFLP 指纹图谱分析检测。PCR 和 Southern 杂交均未从土壤基因组 DNA 中检测到 NDPK2 基因，表明在田间未发生基因转移。此外，连续接种和 AFLP 分析基因组 DNA 后，发现各代疫霉菌的致病性和外观变异没有差异。因此，尽管需要进一步的研究来得出明确的结论，但这些数据表明，尽管存在持续的研究努力，但转基因马铃薯对土壤微生物群落的出现和变异的影响并不显著。
