测量磷脂酸磷酸水解酶活性时遇到的问题。
Problems encountered in measuring the activity of phosphatidate phosphohydrolase.
作者信息
Sturton R G, Brindley D N
出版信息
Biochem J. 1978 Apr 1;171(1):263-6. doi: 10.1042/bj1710263.
Abstract
The measurement of phosphate release from phosphatidate overestimates the microsomal activity of phosphatidate phosphohydrolase from rat liver, since phosphate is also produced via the glycerol phosphate that results from the deacylation of phosphatidate. The determination of phosphate production can be a reliable assay for the soluble phosphatidate phosphohydrolase in rat liver, because the glycerol phosphate formed is not hydrolysed under the conditions used.
摘要
从磷脂酸中释放磷酸盐的测量高估了大鼠肝脏中磷脂酸磷酸水解酶的微粒体活性，因为磷酸盐也通过磷脂酸脱酰基产生的磷酸甘油生成。磷酸盐生成的测定对于大鼠肝脏中的可溶性磷脂酸磷酸水解酶可能是一种可靠的测定方法，因为在所用条件下形成的磷酸甘油不会被水解。
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