HLA-DRB103 和 DRB104 与意大利人群的萎缩性胃炎相关。

HLA-DRB103 and DRB104 are associated with atrophic gastritis in an Italian population.

机构信息

Digestive and Liver Disease Unit, 2nd Medical School, Sapienza University, Rome, Italy.

出版信息

Dig Liver Dis. 2010 Dec;42(12):854-9. doi: 10.1016/j.dld.2010.04.011. Epub 2010 Jun 2.

DOI:10.1016/j.dld.2010.04.011

PMID:20627832

Abstract

BACKGROUND

Atrophic gastritis (AG) is often considered an autoimmune disorder and is associated with other autoimmune diseases. HLA-DRB1 alleles are often associated with autoimmune diseases, however HLA-DRB1 genotyping data in AG patients are lacking. The objective of the study was to evaluate the prevalence of HLA-DRB1 in AG patients.

METHODS

The occurrence of HLA-DRB1 alleles was assessed in 89 Italian AG patients (69.1% female) and 313 controls (47.3% females). Genomic DNA was extracted from peripheral venous blood, PCR-coamplified for HLA-DRB1 and typed using a reverse line-blot assay.

RESULTS

Compared to controls, prevalence of HLA-DRB103 (28.1% vs. 15.9%, p=0.01) and HLA-DRB104 (25.8% vs. 14.4%, p=0.01) was greater in AG patients, conferring an OR of 2.05 and 2.07, respectively. HLA-DRB101 occurred more frequently in controls than in AG patients (11.5% vs. 3.4%, p=0.01) conferring an OR of 0.27. AG patients carrying the HLA-DRB103 or *04 alleles were characterised by having more frequently autoimmune thyroid disease (70.4% vs. 42.2%, p=0.01) and intestinal metaplasia (86.4% vs. 62.2%, p=0.01).

CONCLUSIONS

In our population, over 50% of AG patients carry the HLA-DRB1*03 or *04 alleles associated with autoimmune diseases, suggesting that this subset of AG patients has a genetic predisposition to autoimmunity.

摘要

背景

萎缩性胃炎（AG）常被认为是一种自身免疫性疾病，与其他自身免疫性疾病有关。HLA-DRB1 等位基因常与自身免疫性疾病相关，但 AG 患者的 HLA-DRB1 基因分型数据尚缺乏。本研究旨在评估 AG 患者 HLA-DRB1 的患病率。

方法

评估了 89 例意大利 AG 患者（69.1%为女性）和 313 名对照者（47.3%为女性）中 HLA-DRB1 等位基因的发生情况。从外周静脉血中提取基因组 DNA，PCR 共同扩增 HLA-DRB1 并使用反向线印迹分析进行分型。

结果

与对照组相比，AG 患者 HLA-DRB103（28.1%比 15.9%，p=0.01）和 HLA-DRB104（25.8%比 14.4%，p=0.01）的患病率更高，OR 值分别为 2.05 和 2.07。HLA-DRB101 在对照组中的发生率高于 AG 患者（11.5%比 3.4%，p=0.01），OR 值为 0.27。携带 HLA-DRB103 或 *04 等位基因的 AG 患者更常发生自身免疫性甲状腺疾病（70.4%比 42.2%，p=0.01）和肠化生（86.4%比 62.2%，p=0.01）。