Tang Y, Mennander A, Oksala N, Atalay M, Syvala H, Lagerstedt A, Kuuslahti M, Nieminen R, Anas N, Moilanen E, Tarkka M
Department of Cardiothoracic Surgery, Nanchang University Hospital, Jiangxi, China.
Eur Surg Res. 2010;45(1):1-8. doi: 10.1159/000315479. Epub 2010 Jul 13.
Global warm ischemia of transplanted cardiac grafts is associated with apoptosis and subsequent tissue necrosis. It is suggested that postconditioning (PostC) may ameliorate the early outcome of cardiac grafts after ischemia-reperfusion. We investigated whether PostC and remote postconditioning (RPostC) have a histopathologic impact after transplantation of warm ischemic rat cardiac grafts.
Thirteen rats were randomized to undergo either PostC or RPostC after heterotopic transplantation of ischemic cardiac grafts. Six rats without intervention besides transplantation served as controls (control). The recipient rats were sacrificed 24 h after transplantation to evaluate histopathology and apoptotic index.
No significant differences were observed between the study groups in terms of graft heat shock protein 70 and oxygen radical absorbing capacity, an indicator of antioxidant capacity. While apoptosis and PCARB, a marker of protein oxidation and oxidative stress, decreased after RPostC (p < 0.05), contraction band necrosis was less prominent in both PostC and RPostC.
Both PostC and RPostC have a histopathologic impact after 24 h of reperfusion of warm ischemic rat cardiac grafts.
移植心脏移植物的全身性热缺血与细胞凋亡及随后的组织坏死相关。有研究表明，缺血后处理（PostC）可能改善心脏移植物缺血再灌注后的早期转归。我们研究了热缺血大鼠心脏移植物移植后，缺血后处理和远隔缺血后处理（RPostC）是否具有组织病理学影响。
13只大鼠在异位移植缺血心脏移植物后被随机分为接受缺血后处理或远隔缺血后处理两组。6只除移植外未接受干预的大鼠作为对照组（对照）。移植后24小时处死受体大鼠，以评估组织病理学和凋亡指数。
研究组之间在移植物热休克蛋白70和氧自由基吸收能力（抗氧化能力指标）方面未观察到显著差异。虽然远隔缺血后处理后细胞凋亡及蛋白质氧化和氧化应激标志物PCARB降低（p<0.05），但缺血后处理组和远隔缺血后处理组的收缩带坏死均不明显。
热缺血大鼠心脏移植物再灌注24小时后，缺血后处理和远隔缺血后处理均具有组织病理学影响。
