Tanyeri Melikhan, Kennedy Ian M
Sens Lett. 2008 Apr;6(2):326-329. doi: 10.1166/sl.2008.046.
Detection of single bacterial cells through optical resonances has been demonstrated in microdroplets. The setup enables high throughput non-specific detection of single E. Coli cells without any labeling. The cells inside the microdroplet have a direct effect on the morphology dependent resonances that are supported by Rhodamine 6G fluorescence; modification of the resonances arises from changes due to scattering and changes in the local refractive index. The change in the optical resonance spectrum can be observed at low concentrations where each microdroplet contains no more than one cell.
通过光学共振检测单个细菌细胞已在微滴中得到证实。该装置能够对单个大肠杆菌细胞进行高通量非特异性检测，无需任何标记。微滴内的细胞对罗丹明6G荧光所支持的形态依赖共振有直接影响；共振的改变源于散射引起的变化以及局部折射率的变化。在每个微滴中细胞数量不超过一个的低浓度情况下，即可观察到光学共振光谱的变化。
