Zhou Yong, Yang Jie, Zhao Hua, Li Heng-Jin
Department of Dermatology, General Hospital of PLA, Beijing 100853, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2010 May;41(3):464-6.
To investigate the effect of all-trans retinoic acid on the proliferation of and Fas protein expression in malignant melanoma A375 cells in vitro.
Malignant melanoma A375 cells cultured under different doses of all-trans retinoic acid (ATRA) and solvent controls. The effect of ATRA on cell proliferation was observed. The growth rate of the cells was detected by MTT assay. The Fas protein expression was detected by Western blotting.
ATRA inhibited the proliferation of A375 cells under 1-100 micromol/L of concentrations. The peak effect occurred after 72 hours of ATRA treatment, ATRA inhibited the growth of A375 cells in a dose and time-dependent manner. The level of Fas protein was up-regulated after exposure to 10 micromol/L of ATRA.
ATRA inhibits A375 cell proliferation perhaps through Fas death receptor pathway which induces A375 cell apoptosis.
探讨全反式维甲酸对体外培养的恶性黑色素瘤A375细胞增殖及Fas蛋白表达的影响。
将恶性黑色素瘤A375细胞在不同剂量的全反式维甲酸（ATRA）及溶剂对照下培养。观察ATRA对细胞增殖的影响。采用MTT法检测细胞生长速率。采用蛋白质免疫印迹法检测Fas蛋白表达。
在1-100微摩尔/升浓度下，ATRA抑制A375细胞增殖。ATRA处理72小时后出现最大效应，ATRA以剂量和时间依赖性方式抑制A375细胞生长。暴露于10微摩尔/升的ATRA后，Fas蛋白水平上调。
ATRA可能通过Fas死亡受体途径抑制A375细胞增殖，从而诱导A375细胞凋亡。
