调控蛋白 Yap1 参与酵母 Saccharomyces cerevisiae 对硝化应激的反应。
Regulatory protein Yap1 is involved in response of yeast Saccharomyces cerevisiae to nitrosative stress.
机构信息
Department of Biochemistry, Vassyl Stefanyk Precarpathian National University, Ivano-Frankivsk, 76025, Ukraine.
出版信息
Biochemistry (Mosc). 2010 May;75(5):629-64. doi: 10.1134/s0006297910050135.
The goal of this work was to investigate the possible involvement of protein transcription factor Yap1 in regulation of activity of antioxidant enzymes superoxide dismutase and catalase during yeast response to nitrosative stress. It was found that the inactivation of the YAP1 gene, encoding Yap1p, cancelled the activation of superoxide dismutase and catalase by NO-donors. Then, using chimeric protein Yap1-GFP, we found the accumulation of Yap1p in the nucleus in response to nitrosative stress. Therefore, we conclude that these results in combination with previous data clearly demonstrate the involvement of Yap1p in upregulation of superoxide dismutase and catalase in yeast cells in response to nitrosative stress.
本工作旨在研究转录因子 Yap1 是否参与调控酵母细胞应对硝化应激时抗氧化酶超氧化物歧化酶和过氧化氢酶的活性。结果发现，失活编码 yap1p 的 YAP1 基因，会消除 NO 供体对超氧化物歧化酶和过氧化氢酶的激活作用。然后，我们使用嵌合蛋白 yap1-GFP 发现，硝化应激时 yap1p 会在核内积累。因此，我们得出结论，这些结果与之前的数据一起清楚地表明，硝化应激时 yap1p 参与了酵母细胞中超氧化物歧化酶和过氧化氢酶的上调。
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