Aquatic Animal Health Division, National Research Institute of Aquaculture, Fisheries Research Agency, Mie 516-0193, Japan.
Fish Shellfish Immunol. 2010 Oct;29(4):687-93. doi: 10.1016/j.fsi.2010.07.012. Epub 2010 Jul 13.
We evaluated the tissue persistence and live vaccine efficacy of five avirulent Edwardsiella tarda strains (E22, SU100, SU117, SU138, and SU244) isolated from the Japanese eel (Anguilla japonica) and from the environment. The live vaccines, containing a single strain, were injected intraperitoneally into Japanese flounder (Paralichthys olivaceus). Viable bacteria from all the strains (excluding SU100) were recovered from trunk-kidney tissue 28 d post-injection. Japanese flounder inoculated with E22 had the highest relative percentage survival (RPS = 45%) in an artificial challenge with virulent E. tarda (NUF806). The serum of E22-vaccinated fish had a significantly higher agglutination titer against NUF806. In contrast, there was little or no increase in the agglutination titer of the fish that were inoculated with the remaining avirulent strains. Injection with avirulent E. tarda increased the expression of cytokine genes, including interleukin-1beta (IL-1beta), type 1 interferon (IFN), and IFN-gamma in head-kidney of the Japanese flounder.
我们评估了从日本鳗鲡（Anguilla japonica）和环境中分离的 5 株无致病性爱德华氏菌（E22、SU100、SU117、SU138 和 SU244）的组织持久性和活疫苗效力。将含有单一菌株的活疫苗经腹腔注射入日本牙鲆（Paralichthys olivaceus）体内。在注射后 28 天，所有菌株（SU100 除外）都从肾脏组织中回收了活菌。在人工攻毒实验中，用高致病性爱德华氏菌（NUF806）感染 E22 免疫的日本牙鲆的相对存活率（RPS = 45%）最高。E22 疫苗接种鱼的血清对 NUF806 的凝集效价显著升高。相比之下，感染其他无致病性菌株的鱼的凝集效价几乎没有增加或没有增加。注射无致病性爱德华氏菌可增加日本牙鲆头肾中细胞因子基因（包括白细胞介素-1β（IL-1β）、I 型干扰素（IFN）和 IFN-γ）的表达。
