Hayashi J I, Yonekawa H, Gotoh O, Motohashi J, Tagashira Y
Cancer Lett. 1978 Mar;4(3):125-30. doi: 10.1016/s0304-3835(78)93907-1.
Rat mitochondrial DNAs (mtDNAs) of ascites hepatoma (AH-130) and normal liver cells (Donryu strain) were digested by various restriction endonucleases and the cleavage patterns compared by agarose gel electrophoresis. Different cleavage patterns were observed between AH-130 and liver mtDNAs when they were digested by HindII and EcoRI. The mtDNA of AH-130 lost one clevage site of HindII and one clevage site of EcoRI. The cleavage patterns of mtDNAs from other organs and strains tested were the same as that of liver mtDNA. From these observations we concluded that the molecular clone of AH-130 mtDNA was different from that of other mtDNAs.
用各种限制性内切酶消化腹水肝癌（AH - 130）和正常肝细胞（Donryu品系）的大鼠线粒体DNA（mtDNA），并通过琼脂糖凝胶电泳比较其切割模式。当用HindII和EcoRI消化时，观察到AH - 130和肝脏mtDNA之间存在不同的切割模式。AH - 130的mtDNA失去了一个HindII切割位点和一个EcoRI切割位点。所测试的其他器官和品系的mtDNA切割模式与肝脏mtDNA相同。从这些观察结果我们得出结论，AH - 130 mtDNA的分子克隆与其他mtDNA不同。
