Suter M M, Pantano D M, Flanders J A, Augustin-Voss H G, Dougherty E P, Varvayanis M
Department of Pathology, New York State College of Veterinary Medicine, Cornell University, Ithaca.
Vet Pathol. 1991 Mar;28(2):131-8. doi: 10.1177/030098589102800205.
Neoplastic canine keratinocytes derived from a spontaneous oral squamous cell carcinoma were maintained in culture for more than 45 passages. The presence of desmosomes and keratin filaments was demonstrated by electron microscopy and immunohistochemistry. The keratinocytes were grown in two different culture conditions to induce variations in the stage of differentiation, i.e., in submerged cultures and at the air-liquid interface. For comparison, normal canine keratinocytes were grown under the same conditions. Anisocytosis was present in neoplastic cultures grown submerged in medium. Grown at the air-liquid interface, neoplastic keratinocytes differentiated into a well-organized, multilayered stratified squamous epithelium analogous to normal keratinocytes. Rare areas of irregular growth and formation of whorls were detected. Expression of lectin binding sites and specific cell surface antigens of neoplastic and normal keratinocytes demonstrated marked similarities between the two cell lines. Neoplastic cells lacked certain surface antigens that are present on normal cells. Squamous cell carcinoma cells grew faster than normal canine keratinocytes as demonstrated by growth curve evaluation. Neoplastic keratinocytes responded to growth stimulation by epidermal growth factor and cholera toxin as do normal keratinocytes. Neoplastic cells grown in medium lacking these factors proliferated faster than growth factor stimulated normal keratinocytes.
从自发性口腔鳞状细胞癌中获取的肿瘤性犬角质形成细胞在培养中传代超过45次。通过电子显微镜和免疫组织化学证实了桥粒和角蛋白丝的存在。角质形成细胞在两种不同的培养条件下生长，以诱导分化阶段的变化，即在浸没培养和空气-液体界面培养。作为对照，正常犬角质形成细胞在相同条件下生长。浸没在培养基中生长的肿瘤性培养物中存在细胞大小不均。在空气-液体界面生长时，肿瘤性角质形成细胞分化为组织良好的多层复层鳞状上皮，类似于正常角质形成细胞。检测到罕见的不规则生长区域和漩涡形成。肿瘤性和正常角质形成细胞的凝集素结合位点和特定细胞表面抗原的表达显示这两种细胞系之间有明显的相似性。肿瘤细胞缺乏正常细胞上存在的某些表面抗原。生长曲线评估表明，鳞状细胞癌细胞比正常犬角质形成细胞生长得更快。肿瘤性角质形成细胞与正常角质形成细胞一样，对表皮生长因子和霍乱毒素的生长刺激有反应。在缺乏这些因子的培养基中生长的肿瘤细胞比生长因子刺激的正常角质形成细胞增殖得更快。
