Department of Radiotherapy and Oncological Therapy, Clinic of Haematology and Oncology, Tartu University Hospital, Estonia.
Int J Radiat Biol. 2010 Oct;86(10):860-6. doi: 10.3109/09553002.2010.487025.
A significant reduction in the incidence of radiation-induced oral mucositis by Palifermin has been demonstrated. The underlying mechanisms, however, remain unclear. The aim of the present study was to assess the effect of Palifermin on inflammatory and immune processes during fractionated irradiation in mouse tongue.
Fractionated irradiation, 10 x 3 Gy in two weeks, was given to the snout of the animals. In one group, a single injection of Palifermin (15 mg/kg, s.c.) was given one day before the onset of radiotherapy. Groups of mice (n = 3) were sacrificed from day 1-16 after the start of irradiation. Vasodilatation, endothelial expression of intercellular adhesion molecule 1 (ICAM-1) and the number of CD105-positive (CD105(+)) macrophages were assessed.
Compared to untreated control tissue, irradiation resulted in a significant vasodilatation and an increase in endothelial ICAM-1 staining intensity during the entire study period. Additionally, a significant increase in the number of CD105(+) macrophages was detected. In contrast, with Palifermin treatment before irradiation, none of these changes were found within the first 10 days.
Palifermin pre-treatment resulted in a long-lasting inhibition of radiation-induced inflammatory and immune changes in mouse tongue. This may contribute to the protective effect of this growth factor.
已有研究证实，培洛昔芬可显著降低放射性口腔黏膜炎的发生率。但其潜在机制尚不清楚。本研究旨在评估培洛昔芬对小鼠舌部分次照射过程中炎症和免疫过程的影响。
对动物鼻部进行两周内 10 次 3 Gy 的分次照射。其中一组在放射治疗开始前一天给予单次培洛昔芬（15mg/kg，皮下注射）。从照射开始后第 1 天到第 16 天，每组处死 3 只小鼠。评估血管扩张、细胞间黏附分子 1（ICAM-1）在内皮细胞的表达以及 CD105 阳性（CD105(+)）巨噬细胞的数量。
与未经处理的对照组组织相比，照射导致整个研究期间血管显著扩张，内皮细胞 ICAM-1 染色强度增加。此外，还检测到 CD105(+)巨噬细胞数量的显著增加。相比之下，在照射前用培洛昔芬预处理，则在前 10 天内未发现这些变化。
培洛昔芬预处理可长期抑制小鼠舌部放射诱导的炎症和免疫变化。这可能有助于该生长因子的保护作用。
