Nagahata H, Kojima N, Higashitani I, Ogawa H, Noda H
Department of Animal Health, School of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan.
Zentralbl Veterinarmed B. 1991 Feb;38(1):49-54. doi: 10.1111/j.1439-0450.1991.tb00845.x.
Lymphocyte blastogenic response, B-lymphocyte population and antibody-producing activity of lymphocytes were determined to evaluate the lymphocyte function in neonatal calves during the first 4 weeks of life. The mean percentage of B-lymphocytes ranged from 10.2 to 12.5% during the first 14 days of life and from 15.3 to 17.5% in calves from the day 21 to day 28 after birth. The absolute number of B-lymphocytes increased significantly (P less than 0.05) from 370/microliters at birth to 736/microliters on day 28 after birth. The mean stimulation index of blastogenic response, measured by fluorometric assay, ranged from 5.75 to 6.61 with Con A, from 5.29 to 5.98 with PHA and from 1.89 to 2.50 with PWM. The mean (+/- S.D.) number of plaque forming cells ranged from 22.0 (+/- 12.0) to 24.7 (+/- 9.2) in cultured lymphocytes from 5 calves at birth to 14 days after birth and their levels increased markedly from 137.8 (+/- 88.3) to 162.0 (+/- 57.8) in lymphocytes from 20 days to 28 days after birth. The present study showed that antibody-producing activity of lymphocytes is lower in calves within 3 weeks after birth compared to that of calves 3 weeks after birth, indicating that neonatal calf lymphocytes have a low antibody-producing activity at least up to 1 month after birth.
测定淋巴细胞的成淋巴细胞反应、B淋巴细胞群体和淋巴细胞的抗体产生活性，以评估新生犊牛出生后前4周的淋巴细胞功能。出生后前14天，B淋巴细胞的平均百分比在10.2%至12.5%之间，出生后第21天至第28天，犊牛的B淋巴细胞平均百分比在15.3%至17.5%之间。B淋巴细胞的绝对数量从出生时的370/微升显著增加（P小于0.05）至出生后第28天的736/微升。通过荧光测定法测得的成淋巴细胞反应的平均刺激指数，使用刀豆蛋白A时为5.75至6.61，使用植物血凝素时为5.29至5.98，使用美洲商陆有丝分裂原时为1.89至2.50。出生至出生后14天，5头犊牛培养淋巴细胞中形成空斑细胞的平均（±标准差）数量在22.0（±12.0）至24.7（±9.2）之间，出生后20天至28天，淋巴细胞中形成空斑细胞的水平从137.8（±88.3）显著增加至162.0（±57.8）。本研究表明，与出生3周后的犊牛相比，出生后3周内的犊牛淋巴细胞的抗体产生活性较低，这表明新生犊牛淋巴细胞至少在出生后1个月内抗体产生活性较低。
