[川崎病患儿纤维蛋白原Bβ-148C/T基因多态性与冠状动脉病变的关系]
[Relationship between fibrinogen Bß-148C/T polymorphism and coronary artery lesions in children with Kawasaki disease].
作者信息
Gao Jie, Wang Hong-Yang, Wu Nai-Jun, Zhang Shu-Hua
机构信息
Department of Pediatrics, Affiliated Hospital of North China Coal Medical College, Tangshan, Hebei, China.
出版信息
Zhongguo Dang Dai Er Ke Za Zhi. 2010 Jul;12(7):518-20.
OBJECTIVE
To study the possible relationship between coronary artery lesions and fibrinogen Bbeta-148 C/T polymorphism in children with Kawasaki disease.
METHODS
Fast blood samples were taken from 36 children with Kawasaki disease (21 had coronary artery lesions) and 49 age- and gender-matched healthy children (control group). Plasma levels and molecular reactivity of fibrinogen were measured with Assist Plasma Fibrinogen Activity Assay System. Polymerize chain reaction and restriction enzyme digestion were used to detect the genotypes of fibrinogen Bbeta-148C/T gene polymorphism.
RESULTS
The plasma fibrinogen levels in patients with coronary artery lesions were significantly higher than those in patients without coronary artery lesions and in the control group. T allele frequency in patients with Kawasaki disease was significantly higher than that in the control group. The patients with coronary artery lesions had more increased T allele frequency compared with the patients without coronary artery lesions.
CONCLUSIONS
Plasma fibrinogen levels and fibrinogen Bbeta-148C/T polymorphism are associated with coronary artery lesions in children with Kawasaki disease.
目的
研究川崎病患儿冠状动脉病变与纤维蛋白原Bβ-148 C/T基因多态性之间的可能关系。
方法
采集36例川崎病患儿（其中21例有冠状动脉病变）及49例年龄、性别匹配的健康儿童（对照组）的空腹血样。采用辅助血浆纤维蛋白原活性检测系统测定纤维蛋白原的血浆水平和分子反应性。采用聚合酶链反应和限制性内切酶消化法检测纤维蛋白原Bβ-148C/T基因多态性的基因型。
结果
有冠状动脉病变的患者血浆纤维蛋白原水平显著高于无冠状动脉病变的患者及对照组。川崎病患者的T等位基因频率显著高于对照组。与无冠状动脉病变的患者相比，有冠状动脉病变的患者T等位基因频率升高更为明显。
结论
血浆纤维蛋白原水平及纤维蛋白原Bβ-148C/T基因多态性与川崎病患儿冠状动脉病变有关。
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