Department of Plant Pathology, Shandong Agricultural University, Tai'an 271018, China.
Biotechnol Lett. 2010 Nov;32(11):1719-23. doi: 10.1007/s10529-010-0354-1. Epub 2010 Jul 18.
A new monoamine oxidase type C-like dehydratase gene (MaoC) supplying (R)-3-hydroxyacyl-CoA from the fatty acid oxidation pathway to polyhydroxyalkanoates (PHAs) biosynthetic pathways was identified from Phytophthora capsici. MaoC was over-expressed in Escherichia coli and the recombinant MaoC was purified. The purified tagged MaoC shows the enoyl-CoA hydratase activity of 58 U/mg towards crotonyl-CoA. MaoC may not fold properly above 40°C which was revealed by circular dichroism analysis. Crystal of MaoC diffracts to 1.93 with unit-cell parameters of a = 81.458 Å, b = 82.614 Å, c = 124.228 [corrected] Å, α = β = γ = 90°.
从辣椒疫霉中鉴定出一种新型单胺氧化酶 C 样脱水酶基因（MaoC），该基因能够将脂肪酸氧化途径中的（R）-3-羟酰基辅酶 A 供应给聚羟基烷酸（PHA）生物合成途径。MaoC 在大肠杆菌中过表达，并对重组 MaoC 进行了纯化。纯化的标记 MaoC 对丁烯酰辅酶 A 的烯酰辅酶 A 水合酶活性为 58 U/mg。圆二色性分析表明，MaoC 在 40°C 以上可能无法正确折叠。MaoC 的晶体衍射至 1.93，其晶胞参数为 a = 81.458 Å, b = 82.614 Å, c = 124.228 [校正] Å, α = β = γ = 90°。
