Ijaz Aamir, Khan Iqbal Muhammad, Saeed Farrukh, Tariq Khalid Mahmood
Department Of Pathology, Combined Military Hospital, Peshawar.
J Coll Physicians Surg Pak. 2010 Jun;20(6):405-7.
To measure the effects of awareness campaign on the frequency of EDTA contamination.
A cohort study.
The study was carried out in Combined Military Hospital, Peshawar, from October to December 2008.
A cohort of 200 doctors, nurses and paramedical staff involved in sample collection for laboratory investigations were selected for this three phased study. In all samples with plasma potassium level > 6.0 mmol/L, plasma Ca++ was measured for one month before, during and after a campaign of correct filling order of sample tubes for two weeks. Renal function test and LDH were also measured to rule out chronic renal failure and in-vitro haemolysis, respectively. Fisher's exact test was used for comparison of frequency of hyperhalemia.
There was a significant decrease (75%, p < 0.01) in the cases of spurious hyperkalaemia and hypocalcaemia.
EDTA contamination is a common and important source of pre-analytical error which can be prevented to some extent by education of medical and nursing staff.
评估宣传活动对乙二胺四乙酸（EDTA）污染频率的影响。
队列研究。
2008年10月至12月在白沙瓦联合军事医院开展本研究。
选取参与实验室检查样本采集的200名医生、护士和辅助医疗人员组成队列，进行这项分三个阶段的研究。在为期两周关于正确填写样本管顺序的宣传活动前、活动期间及活动后一个月，对所有血浆钾水平>6.0 mmol/L的样本测量血浆钙离子（Ca++）水平。还分别测量肾功能试验和乳酸脱氢酶（LDH）以排除慢性肾衰竭和体外溶血。采用Fisher精确检验比较高钙血症的频率。
假性高钾血症和低钙血症病例显著减少（75%，p<0.01）。
EDTA污染是分析前误差的常见且重要来源，通过对医护人员进行教育可在一定程度上预防。
