Faculty of Life Science and Biotechnology, Ningbo University, Ningbo, Zhejiang Province 315211, China.
Prostaglandins Other Lipid Mediat. 2010 Nov;93(3-4):93-9. doi: 10.1016/j.prostaglandins.2010.07.001. Epub 2010 Jul 17.
Prostaglandins F2α levels increase during ovulatory period in Xenopus laevis in response to stimulation by gonadotropins and progesterone. PGF2α exerts its effects on ovulation through interaction with its receptor (FP) in ovaries. Little is known about the characteristics of the FP receptor and its regulation during the ovulatory period in non-mammalian species. In the present study, two isoforms of prostaglandin F receptor (FP A and B) cDNAs were isolated from Xenopus laevis ovarian tissues using reverse transcription-polymerase chain reaction (RT-PCR) followed by rapid amplification of cDNA ends (RACE). The cDNAs of FP A and FP B were sequenced. In Xenopus laevis ovary, FP A and B mRNA levels were up-regulated during gonadotropin- and progresterone-induced ovulation in vitro. The mRNA level of FP B was higher than that of FP A. Moreover, FP A and FP B mRNA levels were measured in various tissues including eye, liver, lungs, heart, muscle, ovary, and skin. Overall, FP B mRNA level was approximately 10- to 100-fold higher than that of FP A, except in the muscle and skin where FP A mRNA level was comparable to that of FP B. The results suggest that in Xenopus ovarian follicles FP receptors play an important role during gonadotropin- and progesterone-induced ovulation.
在爪蟾中，PGF2α 水平在排卵期间会增加，这是对促性腺激素和孕激素刺激的反应。PGF2α 通过与卵巢中的受体（FP）相互作用来发挥排卵作用。关于 FP 受体的特性及其在非哺乳动物物种排卵期间的调节，知之甚少。在本研究中，使用逆转录聚合酶链反应（RT-PCR）和快速扩增 cDNA 末端（RACE）从爪蟾卵巢组织中分离出两种前列腺素 F 受体（FP A 和 FP B）cDNA。对 FP A 和 FP B 的 cDNA 进行测序。在爪蟾卵巢中，FP A 和 FP B mRNA 水平在体外促性腺激素和孕激素诱导的排卵期间上调。FP B 的 mRNA 水平高于 FP A。此外，还测量了 FP A 和 FP B mRNA 在包括眼睛、肝脏、肺、心脏、肌肉、卵巢和皮肤在内的各种组织中的水平。总体而言，FP B mRNA 水平约为 FP A 的 10 到 100 倍，除了肌肉和皮肤中 FP A mRNA 水平与 FP B 相当。这些结果表明，在爪蟾卵巢滤泡中，FP 受体在促性腺激素和孕激素诱导的排卵中发挥重要作用。
