School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Aug 15;878(24):2260-5. doi: 10.1016/j.jchromb.2010.06.016. Epub 2010 Jun 25.
A rapid, sensitive and specific high performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) method has been developed for quantification of mitoxantrone in rat plasma. The analyte and palmatine (internal standard) were extracted from plasma samples with diethyl ether-dichloromethane (3:2, v/v) and separated on a C(18) column. The chromatographic separation was achieved within 2.5min using methanol-10mM ammonium acetate containing 0.1% acetic acid as the mobile phase at a flow rate of 0.2mL/min. The method was linear over the range of 0.5-500ng/mL. The lower limit of quantification (LLOQ) was 0.5ng/mL. Finally, the method was successfully applied to a pharmacokinetic study of mitoxantrone in rats following intravenous administration.
建立了一种快速、灵敏、特异的高效液相色谱-串联质谱（HPLC-MS/MS）法，用于检测大鼠血浆中的米托蒽醌。采用二乙醚-二氯甲烷（3:2，v/v）提取血浆样品中的分析物和延胡索乙素（内标），以甲醇-10mM 乙酸铵含 0.1%乙酸为流动相，在 C（18）柱上进行分离。在 0.2mL/min 的流速下，使用甲醇-10mM 乙酸铵含 0.1%乙酸作为流动相，在 2.5min 内实现了色谱分离。该方法在 0.5-500ng/mL 范围内呈线性。定量下限（LLOQ）为 0.5ng/mL。最后，该方法成功应用于大鼠静脉注射米托蒽醌后的药代动力学研究。
