内部乳胶凝集试验与IgM酶联免疫吸附测定和显微镜凝集试验在钩端螺旋体病诊断中的比较

Comparison of an in-house latex agglutination test with IgM ELISA and MAT in the diagnosis of leptospirosis.

作者信息

Shekatkar S, Acharya N S, Harish B N, Parija S C

机构信息

Department of Microbiology, JIPMER, Pondicherry, India.

出版信息

Indian J Med Microbiol. 2010 Jul-Sep;28(3):238-40. doi: 10.4103/0255-0857.66484.

DOI:10.4103/0255-0857.66484

PMID:20644313

Abstract

The laboratory diagnosis of leptospirosis is fraught with several problems. Isolation of Leptospira by culture has a low sensitivity and the microscopic agglutination test (MAT) is time consuming To overcome these problems, a rapid latex agglutination test (LAT) has been standardized for the detection of antileptospiral antibodies in serum samples from suspected cases of leptospirosis. We compared the efficiency of the LAT to a commercially available IgM ELISA and MAT. A total of 150 serum samples were tested by LAT, IgM ELISA, and MAT. The positivity was 26.7%, 26% and 24% respectively. The sensitivity and specificity of LAT as compared to MAT was 90.62 and 91.96% respectively. Even though LAT and ELISA showed similar results, its rapidity and simplicity made latex agglutination test more suitable as a rapid screening test.

摘要

钩端螺旋体病的实验室诊断存在若干问题。通过培养分离钩端螺旋体的敏感性较低，而显微镜凝集试验（MAT）耗时较长。为克服这些问题，已对一种快速乳胶凝集试验（LAT）进行标准化，用于检测疑似钩端螺旋体病病例血清样本中的抗钩端螺旋体抗体。我们将LAT的效率与市售的IgM酶联免疫吸附测定（ELISA）和MAT进行了比较。总共150份血清样本通过LAT、IgM ELISA和MAT进行检测。阳性率分别为26.7%、26%和24%。与MAT相比，LAT的敏感性和特异性分别为90.62%和91.96%。尽管LAT和ELISA结果相似，但其快速性和简便性使乳胶凝集试验更适合作为一种快速筛查试验。