M.V.P.S. College of Pharmacy, Gangapur Road, Shivaji Nagar, Nashik-422 002, Maharashtra, India.
Pharm Biol. 2010 Jul;48(7):822-7. doi: 10.3109/13880200903283699.
The ischemic brain lesions induced in rats by temporary occlusion of the bilateral common carotid arteries and restoration of blood flow to an ischemic brain region is associated with generation of reactive oxygen species with consequent reperfusion injury.
The present study investigated the neuroprotective potential of Hibiscus rosa sinensis L. (Malvaceae) in a bilateral common carotid artery (BCCA) occlusion model of global cerebral ischemic reperfusion.
The animals underwent 30 min BCCA occlusion and 45 min reperfusion. The methanol extract of H. sinensis (100, 200, 300 mg/kg/day for 6 days, p.o.) was administered 30 min before induction of BCCA occlusion.
The bilateral common carotid artery occlusion resulted in increase in lipid peroxidation, and reduction in superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GSH) activity. The extract attenuated the ischemic reperfusion-induced increase in lipid peroxidation and fall in SOD, CAT, and GSH levels. The cerebral hypoperfusion caused a propensity towards anxiety and was accompanied by deficits of learning and memory. The extract ameliorated anxiety and there was improvement of learning and memory.
The administration of H. sinensis prevented the oxidative stress and the biochemical changes associated with cerebral ischemic reperfusion injury. The mechanism of such protection of H. sinensis may be due to cerebral adaptation, through augmentation of cellular antioxidants such as GSH, SOD and CAT. The results suggest the protective role of H. sinensis in ischemic reperfusion injury.
This study indicates the beneficial role of H. sinensis in cerebrovascular insufficiency states and dementia.
双侧颈总动脉闭塞和恢复缺血脑组织血流引起的大鼠脑缺血性病变与活性氧的产生有关，继而导致再灌注损伤。
本研究探讨了芙蓉（锦葵科）在双侧颈总动脉（BCCA）闭塞模型中的神经保护作用。
动物接受 30 分钟的 BCCA 闭塞和 45 分钟的再灌注。在 BCCA 闭塞诱导前 30 分钟，给予芙蓉甲醇提取物（100、200、300mg/kg/天，共 6 天，口服）。
双侧颈总动脉闭塞导致脂质过氧化增加，超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽还原酶（GSH）活性降低。该提取物减轻了缺血再灌注引起的脂质过氧化增加和 SOD、CAT 和 GSH 水平下降。脑低灌注导致焦虑倾向，并伴有学习和记忆缺陷。提取物改善了焦虑，并且学习和记忆得到了改善。
芙蓉的给药可预防与脑缺血再灌注损伤相关的氧化应激和生化变化。芙蓉这种保护作用的机制可能是由于通过增强细胞抗氧化剂如 GSH、SOD 和 CAT 来实现脑适应。结果表明芙蓉在缺血再灌注损伤中具有保护作用。
本研究表明芙蓉在脑血管功能不全和痴呆状态中具有有益作用。
