验证和评估有性繁殖鱼类仔鱼耳石跨代质量标记的有效性。

Validation and efficacy of transgenerational mass marking of otoliths in viviparous fish larvae.

机构信息

School of Aquatic and Fishery Sciences, University of Washington, 1122 NE Boat Street, Box 355020, Seattle, WA 98105-5020, USA.

出版信息

J Fish Biol. 2010 Jul;77(1):292-8. doi: 10.1111/j.1095-8649.2010.02681.x.

DOI:10.1111/j.1095-8649.2010.02681.x

PMID:20646154

Abstract

Transgenerational mass marking of viviparous fish larvae in vivo was validated by intra-muscular injection of elemental strontium chloride (SrCl(2)) in gestating females and detection of the Sr in the otoliths of developing larvae. All otoliths of brown rockfish Sebastes auriculatus larvae produced from SrCl(2)-injected females showed enriched Sr:Ca ratios near the otolith edges, and the signatures did not appear to be affected by the anterior, centre and posterior positions of larvae within the ovary. Results from the present study indicate that transgenerational marking is a highly reliable technique for marking large numbers of extremely small viviparous fish larvae.

摘要

通过向怀孕的雌鱼肌肉内注射元素氯化锶（SrCl(2)），并在发育中的幼鱼的耳石中检测锶，验证了有胎生鱼类幼鱼的跨代质量标记。来自注射 SrCl(2)的雌鱼的所有褐色石斑鱼 Sebastes auriculatus 幼鱼的耳石都显示出靠近耳石边缘的富集 Sr:Ca 比值，并且这些特征似乎不受幼鱼在卵巢内的前、中、后位置的影响。本研究的结果表明，跨代标记是一种非常可靠的技术，可以标记大量非常小的有胎生鱼类幼鱼。