Universidad Austral de Chile, Instituto de Farmacia, Facultad de Ciencias, Valdivia, Chile.
J Sex Med. 2010 Nov;7(11):3598-609. doi: 10.1111/j.1743-6109.2010.01937.x.
The purpose of this study was to provide a quantitative assessment of female rat sexual behaviors after acute exposure to the A-ring reduced testosterone metabolite, androstanediol (3α-Diol), through the nucleus accumbens (NA) shell.
Quantitative analyses of female rat sexual behaviors and assessment of protein levels for the enzyme glutamic acid decarboxylase isoform 67 (GAD67) and gephyrin, a protein that participates in the clustering of GABA-A receptors in postsynaptic cells, were accomplished.
Female rats were ovariectomized and primed with estrogen and progesterone to induce sexual behaviors. Females received a 3α-Diol infusion via guided cannula that aimed to the NA shell five minutes prior to a sexual encounter with a stud male. The following parameters were videotaped and measured in a frame by frame analysis: lordosis quotient (LQ), Lordosis rating (LR), frequency and duration of proceptive behaviors (hopping/darting and ear wiggling). Levels of GAD67 and gephyrin were obtained by Western blot analysis two or twenty-four hours after the sexual encounter.
Acute exposure to 3α-Diol in the NA shell enhanced LR, ear wiggling, and hopping/darting but not LQ. Some of these behavioral effects were counteracted by co-infusion of 3α-Diol plus the GABAA-receptor antagonist GABAzine. A transient reduction of GAD67 levels in the NA shell was detected.
The testosterone metabolite 3α-Diol enhances sexual proceptivity, but not receptivity, when infused into the NA shell directly. The GABAergic system may participate in the androgen-mediated enhancement of female rat sexual motivation.
本研究旨在通过伏隔核（NA）壳，对急性暴露于 A 环降低的睾酮代谢物雄烷二醇（3α-Diol）的雌性大鼠性行为进行定量评估。
完成了雌性大鼠性行为的定量分析，并评估了谷氨酸脱羧酶同工型 67（GAD67）和 gephyin 的蛋白水平，后者参与了突触后细胞中 GABA-A 受体的聚类。
雌性大鼠被卵巢切除术，并给予雌激素和孕激素预处理以诱导性行为。在与雄性研究对象发生性行为之前五分钟，雌性大鼠通过引导套管接受 3α-Diol 输注。以下参数通过视频记录并逐帧测量：背屈商数（LQ）、背屈评分（LR）、求爱行为（跳跃/猛冲和耳摆动）的频率和持续时间。在性行为发生后两小时或二十四小时通过 Western blot 分析获得 GAD67 和 gephyin 的水平。
急性暴露于 NA 壳中的 3α-Diol 增强了 LR、耳摆动和跳跃/猛冲，但不增强 LQ。这些行为效应中的一些被 3α-Diol 加 GABAA 受体拮抗剂 GABAzine 的共输注所抵消。在 NA 壳中检测到 GAD67 水平的短暂降低。
当将 3α-Diol 注入 NA 壳中时，睾酮代谢物 3α-Diol 增强了雌性大鼠的性前动性，但不增强性接受性。GABA 能系统可能参与了雄性激素介导的雌性大鼠性动机增强。
