Wang Xin, Zhao Tong, Zhao Jia-jia, Xiong Jing-bo
Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
Zhonghua Yi Xue Za Zhi. 2010 May 25;90(20):1399-402.
To investigate the expression level of BRCA1, progesterone receptor A (PRA) and B (PRB) in tissue of sporadic invasive ductal breast carcinoma and further statistically analyze the BRCA1 effects on the expression rates of PRA and PRB.
Sixty-eight cases of adenosis of breast and sporadic invasive ductal breast carcinoma were selected. The corresponding paraffin-embodied tissues were collected from the archive of Department of Pathology, Nanfang Hospital. The expressions level of BRCA1, PRA and PRB were detected by immunohistochemistry. Wilcoxon two-sample test was used to analyze the differential expression of BRCA1 between adenosis and sporadic invasive ductal breast carcinoma. Chi-square test was used to analyze the effects of BRCA1 on the PRA or PRB expression rate. P value < 0.05 was considered statistically significant.
(1) In invasive sporadic ductal breast carcinoma, the positive rate of BRCA1 expression of 60.29% (41/68) was lower than the positive rate of BRCA1 expression at 85.30% (58/68) in adenosis of breast. And the difference of BRCA1 expression between two groups was statistically significant (P < 0.01); (2) In invasive sporadic ductal breast carcinoma, the positive rate of PRA expression for negative BRCA1 expression samples was 81.48% (22/27) and it was higher than the positive rate of PRA expression at 53.66% (22/47) for positive BRCA1 expression samples. And the difference of PRA expression rates between two groups was statistically significant (P < 0.05). It indicated that the expression of BRCA1 affected the expression rate of PRA; In invasive sporadic ductal breast carcinoma, the PRB expression rates between positive and negative BRCA1 expression samples were not statistically significant (P > 0.05). It indicated that BRCA1 had no effect upon the expression rate of PRB.
In sporadic breast carcinoma, a negative expression of BRCA1 is selectively associated with a higher expression rate of PRA rather than PRB. Thus BRCA1 selectively regulates the expression of PRA in sporadic breast carcinoma.
探讨散发性浸润性导管癌组织中BRCA1、孕激素受体A（PRA）和B（PRB）的表达水平，并进一步统计分析BRCA1对PRA和PRB表达率的影响。
选取68例乳腺腺病及散发性浸润性导管癌患者。从南方医院病理科存档中收集相应的石蜡包埋组织。采用免疫组织化学法检测BRCA1、PRA和PRB的表达水平。采用Wilcoxon两样本检验分析乳腺腺病与散发性浸润性导管癌中BRCA1的差异表达。采用卡方检验分析BRCA1对PRA或PRB表达率的影响。P值<0.05认为差异有统计学意义。
（1）在散发性浸润性导管癌中，BRCA1表达阳性率为60.29%（41/68），低于乳腺腺病中BRCA1表达阳性率85.30%（58/68）。两组间BRCA1表达差异有统计学意义（P<0.01）；（2）在散发性浸润性导管癌中，BRCA1表达阴性样本的PRA表达阳性率为81.48%（22/27），高于BRCA1表达阳性样本的PRA表达阳性率53.66%（22/47）。两组间PRA表达率差异有统计学意义（P<0.05）。表明BRCA1的表达影响PRA的表达率；在散发性浸润性导管癌中，BRCA1表达阳性与阴性样本间PRB表达率差异无统计学意义（P>0.05）。表明BRCA1对PRB表达率无影响。
在散发性乳腺癌中，BRCA1的阴性表达与较高的PRA表达率而非PRB表达率选择性相关。因此，BRCA1在散发性乳腺癌中选择性调节PRA的表达。
