一种依赖白细胞介素-6生长的浆细胞白血病细胞系以及一种同时依赖白细胞介素-3和白细胞介素-6的双表型亚克隆的建立与特性分析
Establishment and characterization of a plasma cell leukaemia cell line dependent for growth on IL-6 and a bi-phenotypic subclone dependent upon both IL-3 and IL-6.
作者信息
Kobayashi M, Miyagishima T, Imamura M, Maeda S, Gotohda Y, Iwasaki H, Sakurada K, Miyazaki T
机构信息
Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
出版信息
Br J Haematol. 1991 Jun;78(2):217-21. doi: 10.1111/j.1365-2141.1991.tb04419.x.
A human plasma cell leukaemia cell line (HSM-2) and a subclone (HSM-2.3) have been established from the bone marrow of a patient with bi-phenotypic leukaemia. Proliferation assays using a variety of cytokines demonstrated that HSM-2 proliferated in response to recombinant interleukin-6 (rIL-6), but did not respond to rIL-1, rIL-2, rIL-3, rIL-4, rIL-5, recombinant granulocyte-colony stimulating factor (rG-CSF), or recombinant granulocyte-macrophage-colony stimulating factor (rGM-CSF), and that HSM-2.3 responded to rIL-3 and rIL-6. HSM-2 expressed the CD38 (OKT10), PCA-1, cytoplasmic-IgM, and surface kappa light chain. HSM-2.3 expressed the CD14 (My4), CD33 (My9), CD38 (OKT10), CD19 (B4), CD24 (OKB2), CD10 (J5), PCA-1. HSM-2 and HSM-2.3 are useful tools for analysing the possible role of IL-3 and IL-6 in the oncogenesis of plasma cell leukaemia.
已从一名双表型白血病患者的骨髓中建立了人浆细胞白血病细胞系（HSM - 2）及其亚克隆（HSM - 2.3）。使用多种细胞因子进行的增殖试验表明，HSM - 2对重组白细胞介素 - 6（rIL - 6）有增殖反应，但对rIL - 1、rIL - 2、rIL - 3、rIL - 4、rIL - 5、重组粒细胞集落刺激因子（rG - CSF）或重组粒细胞 - 巨噬细胞集落刺激因子（rGM - CSF）无反应，且HSM - 2.3对rIL - 3和rIL - 6有反应。HSM - 2表达CD38（OKT10）、PCA - 1、细胞质免疫球蛋白M和表面κ轻链。HSM - 2.3表达CD14（My4）、CD33（My9）、CD38（OKT10）、CD19（B4）、CD24（OKB2）、CD10（J5）、PCA - 1。HSM - 2和HSM - 2.3是分析IL - 3和IL - 6在浆细胞白血病肿瘤发生中可能作用的有用工具。
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