Ema M, Iwase T, Iwase Y, Ogawa Y
National Institute of Health Sciences, Osaka Branch, OsakaJapan.
Toxicol In Vitro. 1995 Oct;9(5):703-9. doi: 10.1016/0887-2333(95)00059-h.
Dysmorphogenic effects of di-n-butyltin dichloride (DBTC) were evaluated in vitro using the rat whole embryo culture system. Wistar rat embryos were explanted on day 8 of gestation and cultured for 68 hr. DBTC was added to the culture medium at the start of culture to achieve final concentrations of 3, 10 and 30 ng/ml. At the end of culture, the embryos were examined for vascularization development in the body and yolk sac, yolk sac diameter, crown-rump length, number of somite pairs, morphological score and external anomalies. Significantly and markedly decreased incidences of embryos with well developed vascularization in the body and yolk sac, yolk sac diameter, crown-rump length and number of somite pairs were found at 30 ng/ml. A concentration-dependent decrease in the morphological score and increase in the incidence of embryos with anomalies were noted, and the differences were significant for embryos exposed to 10 and 30 ng/ml DBTC. Open anterior neuropore and craniofacial abnormality were predominantly observed. It is concluded that DBTC possesses dysmorphogenic effects on postimplantation embryos in vitro.
使用大鼠全胚胎培养系统在体外评估二正丁基二氯化锡（DBTC）的致畸作用。在妊娠第8天取出Wistar大鼠胚胎并培养68小时。在培养开始时将DBTC添加到培养基中，以达到最终浓度为3、10和30 ng/ml。培养结束时，检查胚胎的体内和卵黄囊血管化发育、卵黄囊直径、头臀长度、体节对数、形态学评分和外部异常情况。在30 ng/ml时，发现体内和卵黄囊血管化发育良好的胚胎发生率显著且明显降低，卵黄囊直径、头臀长度和体节对数也显著降低。形态学评分呈浓度依赖性降低，异常胚胎的发生率增加，对于暴露于10和30 ng/ml DBTC的胚胎，差异具有统计学意义。主要观察到开放性前神经孔和颅面异常。得出结论，DBTC在体外对植入后胚胎具有致畸作用。
