Department of Analytical Chemistry, Ruhr-University Bochum, Germany.
J Proteomics. 2010 Nov 10;73(12):2336-53. doi: 10.1016/j.jprot.2010.07.006. Epub 2010 Jul 25.
Corynebacterium glutamicum is one of the most important microorganisms because of its ability to produce and secrete glutamate, lysine and other amino acids. To optimize biotechnological amino acid synthesis it is therefore necessary to understand well how metabolic fluxes can be altered by studying the proteins directing these fluxes. In this work we give a comprehensive quantitative outline about the proteomic state of the l-lysine producing mutant strain DM1730 compared to wild type strain ATCC 13032 in the stationary phase of growth. This study comprises 1107 soluble and membrane proteins, of which 908 have been quantified. C. glutamicum DM1730 seems to produce a large amount of lysine even at the expense of various housekeeping functions. Generally, several proteins that are involved in stress response were found to be significantly more abundant, whereas many members of the protein expression machinery are less abundant as well as most proteins involved in cell growth and division and cell envelope synthesis. Extensive l-lysine production causes C. glutamicum to suffer from oxidative stress and iron limitation. Ultimately, a changed lipid composition of C. glutamicum's cell envelope seems to increase its fluidity, which might be related to altered physiology and membrane processes.
谷氨酸棒杆菌是一种非常重要的微生物，因为它能够生产和分泌谷氨酸、赖氨酸和其他氨基酸。因此，为了优化生物技术氨基酸的合成，有必要通过研究指导这些通量的蛋白质，充分了解代谢通量如何被改变。在这项工作中，我们全面地定量描述了 l-赖氨酸生产突变株 DM1730 与野生型菌株 ATCC 13032 在生长静止期的蛋白质组状态。本研究包括 1107 种可溶性和膜蛋白，其中 908 种已被定量。谷氨酸棒杆菌 DM1730 似乎即使以牺牲各种维持功能为代价，也要大量生产赖氨酸。一般来说，发现许多与应激反应有关的蛋白质明显更丰富，而许多参与蛋白质表达机制的成员以及大多数参与细胞生长和分裂以及细胞壁合成的蛋白质都较少。大量生产 l-赖氨酸会导致谷氨酸棒杆菌遭受氧化应激和铁限制。最终，谷氨酸棒杆菌细胞膜的脂质组成的改变似乎增加了其流动性，这可能与改变的生理学和膜过程有关。
