Virology Department, PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, Institute of Tropical Medicine Pedro Kouri, Autopista Novia del Mediodia Km. 6 ½, La Lisa, Havana City, Cuba.
Cytokine. 2010 Dec;52(3):175-83. doi: 10.1016/j.cyto.2010.06.010. Epub 2010 Jul 22.
Dengue virus has become endemic in most tropical urban areas throughout the world, and DHF has appeared concomitantly with this expansion. The intensity of dengue virus replication during the early stages of infection could determine clinical outcomes; therefore, it is important to understand the impact of dengue virus infection on the earliest immune defense against microbial infection, which also strongly regulates the adaptive immune responses. This study was aimed at evaluating the expression of the CC-chemokines MIP-1α/CCL3 and MCP-1/CCL2 in peripheral blood leukocytes using an ex vivo model resembling dengue infection in vivo, in subjects with a well characterized dengue immune background, due to the exceptional Cuban epidemiological situation in dengue. The expression of IFNγ, TNFα and IL10 was also evaluated, giving insight about the role of MCP-1 and MIP-1α in the interplay between innate and adaptive immunity. From individuals with different dengue immune background after dengue virus challenge, increased and different expression of the chemokines and cytokines studied was verified in peripheral blood mononuclear cells, thus demonstrating that the previous immunity to a dengue virus serotype has a strong influence on the early immune response after dengue re-infection.
登革热病毒已在世界上大多数热带城市地区流行，并且登革出血热也随之出现。感染早期登革热病毒的复制强度可能决定临床结局；因此，了解登革热病毒感染对微生物感染的最早免疫防御的影响非常重要，而这种免疫防御也强烈调节适应性免疫反应。本研究旨在评估在具有明确登革热免疫背景的个体中，使用类似于体内登革热感染的体外模型，评估 CC 趋化因子 MIP-1α/CCL3 和 MCP-1/CCL2 在周围血白细胞中的表达，因为在登革热方面，古巴的流行病学情况特殊。还评估了 IFNγ、TNFα 和 IL10 的表达，深入了解 MCP-1 和 MIP-1α 在先天免疫和适应性免疫相互作用中的作用。从登革热病毒攻击后具有不同登革热免疫背景的个体中，在外周血单核细胞中验证了所研究趋化因子和细胞因子的增加和不同表达，从而表明先前对登革热病毒血清型的免疫对再次感染登革热后的早期免疫反应有强烈影响。
