Chu Qin, Zhang Yi, Sun Dong-Xiao, Yu Ying, Wang Ya-Chun, Zhang Yuan
College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Yi Chuan. 2010 Jul;32(7):732-6. doi: 10.3724/sp.j.1005.2010.00732.
Complex vertebral malformation is caused by a single base mutation from G to T at the nucleotide position 559 in the bovine solute carrier family 35 member 3 (SLC35A3) gene exon 4 on Bos Taurus autosome (BTA) 3. The presence of the disease gene in Holstein dairy cattle has been reported in many countries. In this study, we examined 38 top Chinese Holstein sires in Beijing, four of which were identified as CVM carriers. Furthermore, 555 daughters of the four CV sires were examined and 44.0% of them were identified as heterozygotes at the mutation site. The association analysis between estimated breeding values (EBV) of dairy performance traits and the polymorphism showed that there were extremely significant differences between the carriers and the non-carriers (P<0.01). The EBVs of the five milk production traits of CVM carriers were significantly higher than those of non-carriers, and the lactation persistency and somatic cell score (SCS) were also higher in CVM carriers. Therefore, CVM gene seems to link with a QTL or gene associated with milk production traits on BTA3. It is recommended to cull the CVM carriers gradually for economical and breeding reasons.
复杂脊椎畸形是由牛3号常染色体（BTA）上牛溶质载体家族35成员3（SLC35A3）基因外显子4中第559位核苷酸从G到T的单碱基突变引起的。许多国家都报道了荷斯坦奶牛中存在这种致病基因。在本研究中，我们检测了北京的38头顶级中国荷斯坦公牛，其中4头被鉴定为复杂脊椎畸形（CVM）携带者。此外，对这4头CVM公牛的555头女儿进行了检测，其中44.0%在突变位点被鉴定为杂合子。奶牛生产性能性状的估计育种值（EBV）与该多态性之间的关联分析表明，携带者和非携带者之间存在极显著差异（P<0.01）。CVM携带者的五个产奶性状的EBV显著高于非携带者，并且CVM携带者的泌乳持久性和体细胞评分（SCS）也更高。因此，CVM基因似乎与BTA3上一个与产奶性状相关的QTL或基因存在关联。出于经济和育种原因，建议逐步淘汰CVM携带者。
