Liu Chu-Wu, Li Jin-Ming, Liu Li, Guo Yu-Song
Fisheries College, Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Guangdong Ocean University, Zhanjiang 524025, China.
Yi Chuan. 2010 Jul;32(7):737-43. doi: 10.3724/sp.j.1005.2010.00737.
With the construction of a library of partial fractionated genomic DNA of Panulirus stimpsoni Hoehuis, the microsatellite sequences of P. stimpsoni were screened by PCR technique. Then, the genetic diversity was analyzed with the microsatellite markers. Seventy-eight microsatellite sequences in 55 positive recombinant clones were obtained by PCR technique with primers of M13+/- and (CT)15, and (AT)15. Among these microsatellite sequences, the numbers of perfect, imperfect, compound perfect, and compound imperfect sequences were 50 (64%), 3 (3.8%), 5 (7.7%), and 19 (24.5%), respectively. To analyze genomic DNA diversity of P. stimpsoni, 15 pairs of primers were designed from the microsatellite flanking sequences. In these microsatellite loci, the alleles numbers ranged from 3 to 12; and the sizes of these alleles ranged from 78 to 425 bp, which are in accordance with their predicted size range. The expected heterozygosity (He) and the polymorphism information content (PIC) ranged from 0.48 to 0.87 and 0.44 to 0.84 with the average values of 0.71 and 0.60, respectively. These results showed that these microsatellite loci were suitable for P. stimpsoni molecule markers and genetic analysis because of their richness in genetic information.
通过构建中国龙虾部分基因组DNA文库，利用PCR技术筛选中国龙虾的微卫星序列。然后，用微卫星标记分析其遗传多样性。用M13 + / - 引物、(CT)15引物和(AT)15引物通过PCR技术从55个阳性重组克隆中获得了78个微卫星序列。在这些微卫星序列中，完美序列、非完美序列、复合完美序列和复合非完美序列的数量分别为50个(64%)、3个(3.8%)、5个(7.7%)和19个(24.5%)。为分析中国龙虾基因组DNA多样性，从微卫星侧翼序列设计了15对引物。在这些微卫星位点中，等位基因数范围为3至12个；这些等位基因的大小范围为78至425 bp，与预测的大小范围一致。期望杂合度(He)和多态信息含量(PIC)范围分别为0.48至0.87和0.44至0.84，平均值分别为0.71和0.60。这些结果表明，这些微卫星位点由于其丰富的遗传信息，适合作为中国龙虾的分子标记和遗传分析。
