the EH Graham Centre for Agricultural Innovation, Wagga Wagga Agricultural Institute, Wagga Wagga, NSW 2650, Australia.
J Hered. 2010 Nov-Dec;101(6):769-74. doi: 10.1093/jhered/esq083. Epub 2010 Jul 21.
The barley male sterility gene (msg6) located on chromosome 6H has been used in breeding and research since its discovery 7 decades ago, but to date, no research has been reported that linked the gene with molecular markers. The main objective of this study was to identify expressed sequence tag-simple sequence repeat (EST-SSR) markers linked to msg6 as this could provide opportunities for gene discovery. In a cross of a male sterile line (04-042B) with a fully fertile line (VB0330; VB9524/Mundah), male sterility segregated in a 3:1 ratio of fertile to completely sterile plants (χ(2) = 0.03, P(0.05) = 0.95), in a population of 250 F(2) plants. Multipoint linkage mapping placed the msg6 gene at 4.9 cM from the EST-SSR, GBM1267, whereas 2-point analysis estimated a recombination fraction of 0.05 ± 0.02 (logarithm of the odds score = 26.34) between the EST-SSR and the male sterility gene. Multiple interval quantitative trait locus (QTL) analysis of spike weight, an indicative measure of reproductive success, identified a QTL near GBM1267 as having a major influence, explaining 68.7% of the variation in weight of individual spikes. The GBM1267 marker segregated in a 1:2:1 ratio, which makes it highly desirable for marker-assisted selection, as it can distinguish the recessive from the dominant and from heterozygous individuals. Another EST-SSR marker, designated VBMS103, was developed in the present study to provide an additional marker with known sequence (AL501881) close to the msg6 gene. The results provide highly informative functional tools for tracking the msg6 gene in breeding programs.
大麦雄性不育基因（msg6）位于 6H 染色体上，自 70 年前发现以来，一直被用于育种和研究，但迄今为止，尚未有研究将该基因与分子标记联系起来。本研究的主要目的是鉴定与 msg6 连锁的表达序列标签-简单重复序列（EST-SSR）标记，因为这可以为基因发现提供机会。在一个不育系（04-042B）与一个完全可育系（VB0330；VB9524/Mundah）的杂交中，雄性不育在可育植株与完全不育植株之间以 3:1 的比例分离（χ²=0.03，P（0.05）=0.95），在 250 株 F2 植物群体中。多点连锁作图将 msg6 基因定位在距离 EST-SSR GBM1267 4.9cM 的位置，而两点分析估计 EST-SSR 和雄性不育基因之间的重组分数为 0.05±0.02（对数几率得分=26.34）。穗重的多个区间数量性状位点（QTL）分析，穗重是生殖成功的一个指示性衡量指标，鉴定出一个靠近 GBM1267 的 QTL 具有主要影响，解释了个体穗重变异的 68.7%。GBM1267 标记以 1:2:1 的比例分离，这使其非常适合标记辅助选择，因为它可以区分隐性、显性和杂合个体。本研究还开发了另一个 EST-SSR 标记 VBMS103，以提供一个与 msg6 基因附近的已知序列（AL501881）接近的额外标记。这些结果为跟踪育种计划中的 msg6 基因提供了高度信息丰富的功能工具。
