State-Key Laboratory of Biomembrane and Membrane, Biotechnology, School of Life Sciences, Tsinghua University, 100084, Beijing, China.
Nanoscale Res Lett. 2009 Oct 29;5(1):224-30. doi: 10.1007/s11671-009-9469-5.
Purpose of this study was to establish a lecithin nanoemulsion (LNE) without any synthetic surfactant as a topical delivery vehicle and to evaluate its topical delivery potential by the following factors: particle size, morphology, viscosity, stability, skin hydration and skin penetration. Experimental results demonstrated that an increasing concentration of soybean lecithin and glycerol resulted in a smaller size LNE droplet and increasing viscosity, respectively. The droplet size of optimized LNE, with the glycerol concentration above 75% (w/w), changed from 92 (F10) to 58 nm (F14). Additionally, LNE, incorporated into o/w cream, improved the skin hydration capacity of the cream significantly with about 2.5-fold increase when the concentration of LNE reached 10%. LNE was also demonstrated to improve the penetrability of Nile red (NR) dye into the dermis layer, when an o/w cream, incorporated with NR-loaded LNE, applied on the abdominal skin of rat in vivo. Specifically, the arbitrary unit (ABU) of fluorescence in the dermis layer that had received the cream with a NR-loaded LNE was about 9.9-fold higher than the cream with a NR-loaded general emulsion (GE). These observations suggest that LNE could be used as a promising topical delivery vehicle for lipophilic compounds.
本研究旨在建立一种不含任何合成表面活性剂的大豆卵磷脂纳米乳（LNE）作为局部传递载体，并通过以下因素评估其局部传递潜力：粒径、形态、粘度、稳定性、皮肤水合作用和皮肤渗透。实验结果表明，大豆卵磷脂和甘油浓度的增加分别导致 LNE 液滴的粒径更小和粘度增加。在甘油浓度高于 75%（w/w）的情况下，优化后的 LNE 的粒径从 92nm（F10）变为 58nm（F14）。此外，LNE 被掺入到 o/w 乳膏中，可显著提高乳膏的皮肤水合能力，当 LNE 的浓度达到 10%时，水合能力增加约 2.5 倍。当在体内将含有尼罗红（NR）负载 LNE 的 o/w 乳膏施用于大鼠腹部皮肤时，LNE 还被证明可以提高 NR 染料渗透到真皮层的能力。具体而言，接受含有 NR 负载 LNE 的乳膏的真皮层的任意单位（ABU）荧光强度约为含有 NR 负载普通乳液（GE）的乳膏的 9.9 倍。这些观察结果表明，LNE 可作为亲脂性化合物的有前途的局部传递载体。
